The extended packaging sequence of MoMLV contains a constitutive mRNA nuclear export function

Jason A. King, Joanna M. Bridger, Fotini Gounari, Peter Lichter, Thomas F. Schulz, Volker Schirrmacher, Khashayarsha Khazaie

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The present report shows that incorporation of defined sequences from the Moloney murine leukaemia virus (MoMLV) into Rex dependent expression vectors based on the human T-cell leukaemia virus (HTLV-1) allows Rex independent gene expression. Deletion mutagenesis of the MoMLV derived sequences allowed this function to be localised to a 312 nt length sequence overlapping the MoMLV gag p15/p12 open reading frame. This 'extended packaging sequence' has been reported to markedly increase the titre of in vitro packaged retroviral vectors. Using fluorescent in situ hybridisation combined with confocal microscopy we show that the 312 nt element can replace Rex mediated nuclear export and expression of transcripts containing HTLV-1 cis acting repressive elements. Our observations are consistent with the extended packaging sequence of MoMLV exerting a constitutive mRNA nuclear export function. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)367-371
Number of pages5
JournalFEBS Letters
Volume434
Issue number3
DOIs
StatePublished - Sep 4 1998

Keywords

  • Extended packaging sequence
  • Human T-cell leukemia virus-1
  • Moloney murine leukemia virus
  • Rex
  • mRNA nuclear export

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    King, J. A., Bridger, J. M., Gounari, F., Lichter, P., Schulz, T. F., Schirrmacher, V., & Khazaie, K. (1998). The extended packaging sequence of MoMLV contains a constitutive mRNA nuclear export function. FEBS Letters, 434(3), 367-371. https://doi.org/10.1016/S0014-5793(98)00948-X