The expression of proinflammatory cytokine mRNA in the sciatic-tibial nerve of ischemia-reperfusion injury

Yoshiyuki Mitsui; Kunihisa Okamoto; David P. Martin; James D. Schmelzer; Phillip A. Low

doi:10.1016/S0006-8993(99)01830-2

The expression of proinflammatory cytokine mRNA in the sciatic-tibial nerve of ischemia-reperfusion injury

Yoshiyuki Mitsui, Kunihisa Okamoto, David P. Martin, James D. Schmelzer, Phillip A. Low

Neurology

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

We evaluated the proinflammatory cytokines, TNF-α and IL-1β, mRNA expression in the rat sciatic and tibial nerves following ischemia-reperfusion (IR) injury, using competitive RT-PCR, to explore the role of cytokines in IR injury. The expressions of both TNF-α and IL-1β mRNA were related to severity of ischemia and occurred with reperfusion rather than ischemia alone. TNF-α gene expression peaked at 24 h of reperfusion, while that of IL-1β peaked at 12 h. These data support the notion that the proinflammatory cytokines TNF-α and IL-1β are involved in the inflammatory response of IR injury to the peripheral nervous system and may be involved in the pathophysiology of ischemic fiber degeneration. Copyright (C) 1999 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	192-195
Number of pages	4
Journal	Brain Research
Volume	844
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(99)01830-2
State	Published - Oct 9 1999

Keywords

Cytokines
IL-1β
Ischemia
Nerve
Reperfusion
TNF-α

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(99)01830-2

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title = "The expression of proinflammatory cytokine mRNA in the sciatic-tibial nerve of ischemia-reperfusion injury",

abstract = "We evaluated the proinflammatory cytokines, TNF-α and IL-1β, mRNA expression in the rat sciatic and tibial nerves following ischemia-reperfusion (IR) injury, using competitive RT-PCR, to explore the role of cytokines in IR injury. The expressions of both TNF-α and IL-1β mRNA were related to severity of ischemia and occurred with reperfusion rather than ischemia alone. TNF-α gene expression peaked at 24 h of reperfusion, while that of IL-1β peaked at 12 h. These data support the notion that the proinflammatory cytokines TNF-α and IL-1β are involved in the inflammatory response of IR injury to the peripheral nervous system and may be involved in the pathophysiology of ischemic fiber degeneration. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Cytokines, IL-1β, Ischemia, Nerve, Reperfusion, TNF-α",

author = "Yoshiyuki Mitsui and Kunihisa Okamoto and Martin, {David P.} and Schmelzer, {James D.} and Low, {Phillip A.}",

note = "Funding Information: Supported in part by grants from NINDS (NS22352), MDA, and Mayo funds. ",

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AU - Mitsui, Yoshiyuki

AU - Okamoto, Kunihisa

AU - Martin, David P.

AU - Schmelzer, James D.

AU - Low, Phillip A.

N1 - Funding Information: Supported in part by grants from NINDS (NS22352), MDA, and Mayo funds.

PY - 1999/10/9

Y1 - 1999/10/9

N2 - We evaluated the proinflammatory cytokines, TNF-α and IL-1β, mRNA expression in the rat sciatic and tibial nerves following ischemia-reperfusion (IR) injury, using competitive RT-PCR, to explore the role of cytokines in IR injury. The expressions of both TNF-α and IL-1β mRNA were related to severity of ischemia and occurred with reperfusion rather than ischemia alone. TNF-α gene expression peaked at 24 h of reperfusion, while that of IL-1β peaked at 12 h. These data support the notion that the proinflammatory cytokines TNF-α and IL-1β are involved in the inflammatory response of IR injury to the peripheral nervous system and may be involved in the pathophysiology of ischemic fiber degeneration. Copyright (C) 1999 Elsevier Science B.V.

AB - We evaluated the proinflammatory cytokines, TNF-α and IL-1β, mRNA expression in the rat sciatic and tibial nerves following ischemia-reperfusion (IR) injury, using competitive RT-PCR, to explore the role of cytokines in IR injury. The expressions of both TNF-α and IL-1β mRNA were related to severity of ischemia and occurred with reperfusion rather than ischemia alone. TNF-α gene expression peaked at 24 h of reperfusion, while that of IL-1β peaked at 12 h. These data support the notion that the proinflammatory cytokines TNF-α and IL-1β are involved in the inflammatory response of IR injury to the peripheral nervous system and may be involved in the pathophysiology of ischemic fiber degeneration. Copyright (C) 1999 Elsevier Science B.V.

KW - Cytokines

KW - IL-1β

KW - Ischemia

KW - Nerve

KW - Reperfusion

KW - TNF-α

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The expression of proinflammatory cytokine mRNA in the sciatic-tibial nerve of ischemia-reperfusion injury

Abstract

Keywords

ASJC Scopus subject areas

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