The expression of proinflammatory cytokine mRNA in the sciatic-tibial nerve of ischemia-reperfusion injury

Yoshiyuki Mitsui, Kunihisa Okamoto, David P. Martin, James D. Schmelzer, Phillip Anson Low

Research output: Contribution to journalArticle

29 Scopus citations


We evaluated the proinflammatory cytokines, TNF-α and IL-1β, mRNA expression in the rat sciatic and tibial nerves following ischemia-reperfusion (IR) injury, using competitive RT-PCR, to explore the role of cytokines in IR injury. The expressions of both TNF-α and IL-1β mRNA were related to severity of ischemia and occurred with reperfusion rather than ischemia alone. TNF-α gene expression peaked at 24 h of reperfusion, while that of IL-1β peaked at 12 h. These data support the notion that the proinflammatory cytokines TNF-α and IL-1β are involved in the inflammatory response of IR injury to the peripheral nervous system and may be involved in the pathophysiology of ischemic fiber degeneration. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)192-195
Number of pages4
JournalBrain Research
Issue number1-2
StatePublished - Oct 9 1999



  • Cytokines
  • IL-1β
  • Ischemia
  • Nerve
  • Reperfusion
  • TNF-α

ASJC Scopus subject areas

  • Neuroscience(all)

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