The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors

Janet Schaefer-Klein, Iris Givol, Eugene V. Barsov, Jeannette M. Whitcomb, Matthew Vanbrocklin, Douglas N. Foster, Mark J Federspiel, Stephen H. Hughes

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Abstract

The lack of a well-behaved permanent, adherent, nontransformed chicken cell line has made some experiments with avian leukosis-sarcoma viruses (ASLV) and vectors considerably more difficult. The EV-O-derived line, DF-1, supports the efficient replication of subgroups (A), (B), and (C) ASLV, as well as amphotrophic murine leukemia virus and an ASLV-derived vector that has its env gene derived from the env gene from an amphotrophic murine leukemia virus. The cell line responds appropriately to the expression of a transforming oncogene (v-myc) to a growth suppressor gene [p21(waf1)] and can be sorted (using FACS) if infected by an ASLV vector that expresses GFP.

Original languageEnglish (US)
Pages (from-to)305-311
Number of pages7
JournalVirology
Volume248
Issue number2
DOIs
StatePublished - Sep 1 1998

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Alpharetrovirus
env Genes
Murine Leukemia Viruses
Cell Line
myc Genes
Tumor Suppressor Genes
Chickens

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Schaefer-Klein, J., Givol, I., Barsov, E. V., Whitcomb, J. M., Vanbrocklin, M., Foster, D. N., ... Hughes, S. H. (1998). The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors. Virology, 248(2), 305-311. https://doi.org/10.1006/viro.1998.9291

The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors. / Schaefer-Klein, Janet; Givol, Iris; Barsov, Eugene V.; Whitcomb, Jeannette M.; Vanbrocklin, Matthew; Foster, Douglas N.; Federspiel, Mark J; Hughes, Stephen H.

In: Virology, Vol. 248, No. 2, 01.09.1998, p. 305-311.

Research output: Contribution to journalArticle

Schaefer-Klein, J, Givol, I, Barsov, EV, Whitcomb, JM, Vanbrocklin, M, Foster, DN, Federspiel, MJ & Hughes, SH 1998, 'The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors', Virology, vol. 248, no. 2, pp. 305-311. https://doi.org/10.1006/viro.1998.9291
Schaefer-Klein J, Givol I, Barsov EV, Whitcomb JM, Vanbrocklin M, Foster DN et al. The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors. Virology. 1998 Sep 1;248(2):305-311. https://doi.org/10.1006/viro.1998.9291
Schaefer-Klein, Janet ; Givol, Iris ; Barsov, Eugene V. ; Whitcomb, Jeannette M. ; Vanbrocklin, Matthew ; Foster, Douglas N. ; Federspiel, Mark J ; Hughes, Stephen H. / The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors. In: Virology. 1998 ; Vol. 248, No. 2. pp. 305-311.
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