TY - JOUR
T1 - The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors
AU - Schaefer-Klein, Janet
AU - Givol, Iris
AU - Barsov, Eugene V.
AU - Whitcomb, Jeannette M.
AU - Vanbrocklin, Matthew
AU - Foster, Douglas N.
AU - Federspiel, Mark J.
AU - Hughes, Stephen H.
N1 - Funding Information:
We would like to thank Peter K. Vogt for helpful discussions and Hilda Marusiodis for preparing the manuscript. Research was sponsored in part by the National Cancer Institute, Department of Health and Human Services, under contract with ABL (S.H.H.); U.S. Department of Agriculture Competitive Grant 96–35204-3787; Siebens Foundation (under the Harold W. Siebens Scholar Program); Mayo Foundation (M.J.F.); and the Fort Dodge Animal Health, Inc. (Fort Dodge, Iowa) (D.N.F.). The contents of this publication do not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.
PY - 1998/9/1
Y1 - 1998/9/1
N2 - The lack of a well-behaved permanent, adherent, nontransformed chicken cell line has made some experiments with avian leukosis-sarcoma viruses (ASLV) and vectors considerably more difficult. The EV-O-derived line, DF-1, supports the efficient replication of subgroups (A), (B), and (C) ASLV, as well as amphotrophic murine leukemia virus and an ASLV-derived vector that has its env gene derived from the env gene from an amphotrophic murine leukemia virus. The cell line responds appropriately to the expression of a transforming oncogene (v-myc) to a growth suppressor gene [p21(waf1)] and can be sorted (using FACS) if infected by an ASLV vector that expresses GFP.
AB - The lack of a well-behaved permanent, adherent, nontransformed chicken cell line has made some experiments with avian leukosis-sarcoma viruses (ASLV) and vectors considerably more difficult. The EV-O-derived line, DF-1, supports the efficient replication of subgroups (A), (B), and (C) ASLV, as well as amphotrophic murine leukemia virus and an ASLV-derived vector that has its env gene derived from the env gene from an amphotrophic murine leukemia virus. The cell line responds appropriately to the expression of a transforming oncogene (v-myc) to a growth suppressor gene [p21(waf1)] and can be sorted (using FACS) if infected by an ASLV vector that expresses GFP.
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U2 - 10.1006/viro.1998.9291
DO - 10.1006/viro.1998.9291
M3 - Article
C2 - 9721239
AN - SCOPUS:0032168060
SN - 0042-6822
VL - 248
SP - 305
EP - 311
JO - Virology
JF - Virology
IS - 2
ER -