The erasable Western blot

Scott H. Kaufmann, Charles M. Ewing, Joel H. Shaper

Research output: Contribution to journalArticle

138 Scopus citations

Abstract

A method for successfully removing primary and secondary antibodies from nitrocellulose blots while preserving the originally immobilized polypeptides was developed. Polypeptides were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and electrophoretically transferred to nitrocellulose. Nonspecific binding sites were blocked with 5% ( w v) nonfat dried milk. After blots were reacted sequentially with antibodies directed against the antigen of interest and with radiolabeled secondary antibody, a 10-min wash in 5% ( w v) milk was required prior to drying and autoradiography. A 30-min incubation at 70°C in 2% ( w v) sodium dodecyl sulfate containing 100 mm β-mercaptoethanol quantitatively removed the antibodies and allowed reuse of the blot. A modification of this method similarly allowed reuse of Western blots when proteins were immobilized on nylon. Potential applications and limitations of this method are discussed.

Original languageEnglish (US)
Pages (from-to)89-95
Number of pages7
JournalAnalytical Biochemistry
Volume161
Issue number1
DOIs
StatePublished - Feb 15 1987

Keywords

  • antibodies
  • blotting
  • gel electrophoresis
  • immunological methods
  • nuclear envelope polypeptides
  • protein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The erasable Western blot'. Together they form a unique fingerprint.

  • Cite this