The enterohepatic physiology of 24,25-dihydroxyvitamin D3

Rajiv Kumar, S. Nagubandi, J. M. Londowski

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Products of intravenously administered [3H]24,25-dihydroxyvitamin D3 are excreted in the bile of normocalcemia, vitamin D-replete rats. Within 3 and 24 hr, 7.3% ± 1.4 and 18.5 ± 1.4 (mean ± S.D.) of the administered dose appears in the bile. Three hours after the instillation of the biliary products of [3H]24,25-dihydroxyvitamin D3 into the duodena of other rats, 7.6% ± 2.05 of the administered dose appears in newly secreted bile. These data suggest that products of 24,25-dihydroxyvitamin D3 are excreted in bile and undergo an enterohepatic circulation. The metabolites of 24,25-dihydroxyvitamin d3 excreted in bile are much more polar than the parent sterol as assessed by silicic acid chromatography and by high-performance liquid chromatographic techniques. The products are retained on DEAE-cellulose in the presence of methanol are eluted upon the addition of acetic acid to the eluting solvent. The data support the existence of a quantitatively important enterohepatic circulation of polar metabolites of [3H]24,25-dihydroxyvitamin D3; disturbances in this metabolic pathway could help explain the pathogenesis of hepatic and intestinal osteodystrophy.

Original languageEnglish (US)
Pages (from-to)278-284
Number of pages7
JournalJournal of Laboratory and Clinical Medicine
Volume96
Issue number2
StatePublished - 1980

Fingerprint

24,25-Dihydroxyvitamin D 3
Physiology
Bile
Enterohepatic Circulation
Metabolites
Rats
Silicic Acid
DEAE-Cellulose
Sterols
Chromatography
Metabolic Networks and Pathways
Vitamin D
Acetic Acid
Methanol
High Pressure Liquid Chromatography
Liver
Liquids

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

The enterohepatic physiology of 24,25-dihydroxyvitamin D3 . / Kumar, Rajiv; Nagubandi, S.; Londowski, J. M.

In: Journal of Laboratory and Clinical Medicine, Vol. 96, No. 2, 1980, p. 278-284.

Research output: Contribution to journalArticle

Kumar, Rajiv ; Nagubandi, S. ; Londowski, J. M. / The enterohepatic physiology of 24,25-dihydroxyvitamin D3 In: Journal of Laboratory and Clinical Medicine. 1980 ; Vol. 96, No. 2. pp. 278-284.
@article{26ff147a55c54274ad969652ba352228,
title = "The enterohepatic physiology of 24,25-dihydroxyvitamin D3",
abstract = "Products of intravenously administered [3H]24,25-dihydroxyvitamin D3 are excreted in the bile of normocalcemia, vitamin D-replete rats. Within 3 and 24 hr, 7.3{\%} ± 1.4 and 18.5 ± 1.4 (mean ± S.D.) of the administered dose appears in the bile. Three hours after the instillation of the biliary products of [3H]24,25-dihydroxyvitamin D3 into the duodena of other rats, 7.6{\%} ± 2.05 of the administered dose appears in newly secreted bile. These data suggest that products of 24,25-dihydroxyvitamin D3 are excreted in bile and undergo an enterohepatic circulation. The metabolites of 24,25-dihydroxyvitamin d3 excreted in bile are much more polar than the parent sterol as assessed by silicic acid chromatography and by high-performance liquid chromatographic techniques. The products are retained on DEAE-cellulose in the presence of methanol are eluted upon the addition of acetic acid to the eluting solvent. The data support the existence of a quantitatively important enterohepatic circulation of polar metabolites of [3H]24,25-dihydroxyvitamin D3; disturbances in this metabolic pathway could help explain the pathogenesis of hepatic and intestinal osteodystrophy.",
author = "Rajiv Kumar and S. Nagubandi and Londowski, {J. M.}",
year = "1980",
language = "English (US)",
volume = "96",
pages = "278--284",
journal = "Translational Research",
issn = "1931-5244",
publisher = "Mosby Inc.",
number = "2",

}

TY - JOUR

T1 - The enterohepatic physiology of 24,25-dihydroxyvitamin D3

AU - Kumar, Rajiv

AU - Nagubandi, S.

AU - Londowski, J. M.

PY - 1980

Y1 - 1980

N2 - Products of intravenously administered [3H]24,25-dihydroxyvitamin D3 are excreted in the bile of normocalcemia, vitamin D-replete rats. Within 3 and 24 hr, 7.3% ± 1.4 and 18.5 ± 1.4 (mean ± S.D.) of the administered dose appears in the bile. Three hours after the instillation of the biliary products of [3H]24,25-dihydroxyvitamin D3 into the duodena of other rats, 7.6% ± 2.05 of the administered dose appears in newly secreted bile. These data suggest that products of 24,25-dihydroxyvitamin D3 are excreted in bile and undergo an enterohepatic circulation. The metabolites of 24,25-dihydroxyvitamin d3 excreted in bile are much more polar than the parent sterol as assessed by silicic acid chromatography and by high-performance liquid chromatographic techniques. The products are retained on DEAE-cellulose in the presence of methanol are eluted upon the addition of acetic acid to the eluting solvent. The data support the existence of a quantitatively important enterohepatic circulation of polar metabolites of [3H]24,25-dihydroxyvitamin D3; disturbances in this metabolic pathway could help explain the pathogenesis of hepatic and intestinal osteodystrophy.

AB - Products of intravenously administered [3H]24,25-dihydroxyvitamin D3 are excreted in the bile of normocalcemia, vitamin D-replete rats. Within 3 and 24 hr, 7.3% ± 1.4 and 18.5 ± 1.4 (mean ± S.D.) of the administered dose appears in the bile. Three hours after the instillation of the biliary products of [3H]24,25-dihydroxyvitamin D3 into the duodena of other rats, 7.6% ± 2.05 of the administered dose appears in newly secreted bile. These data suggest that products of 24,25-dihydroxyvitamin D3 are excreted in bile and undergo an enterohepatic circulation. The metabolites of 24,25-dihydroxyvitamin d3 excreted in bile are much more polar than the parent sterol as assessed by silicic acid chromatography and by high-performance liquid chromatographic techniques. The products are retained on DEAE-cellulose in the presence of methanol are eluted upon the addition of acetic acid to the eluting solvent. The data support the existence of a quantitatively important enterohepatic circulation of polar metabolites of [3H]24,25-dihydroxyvitamin D3; disturbances in this metabolic pathway could help explain the pathogenesis of hepatic and intestinal osteodystrophy.

UR - http://www.scopus.com/inward/record.url?scp=0018963439&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018963439&partnerID=8YFLogxK

M3 - Article

VL - 96

SP - 278

EP - 284

JO - Translational Research

JF - Translational Research

SN - 1931-5244

IS - 2

ER -