The effects of treatment with chemical agents or infection with feline viruses on protein-binding properties of the feline immunodeficiency virus long terminal repeat

Yasuhiro Ikeda, Yasushi Kawaguchi, Yasuo Inoshima, Mariko Kohmoto, Masayuki Shimojima, Gouki Inada, Eiji Sato, Chieko Kai, Takayuki Miyazawa, Takeshi Mikami

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Abstract

The effects of treatment with chemical agents or infection with feline viruses on protein-binding properties of the feline immunodeficiency virus (FIV) long terminal repeat (LTR) were examined by gel-mobility-shift assays using oligonucleotides designed to represent putative AP-1 or ATF motif from the FIV LTR. Infection with FIV led to less nuclear proteins binding to the AP-1 and ATF sites, suggesting that proteins binding to the sites were consumed or suppressed by FIV-replication in FIV-infected cells. Nuclear proteins that bind to the AP-1 or ATF site were examined by using extracts from Crandell feline kidney (CRFK) cells treated with TPA (a phorbol ester; a strong activator of protein kinase C) or forskolin (an inducer of cyclic-AMP), or infection with feline herpesvirus type 1 (FHV-1). Although TPA or forskolin treatment moderately increased the level of both proteins that bound to AP-1 and ATF sites, FHV-1 infection markedly changed the protein-binding patterns of the sites. Furthermore, FHV-1-induced proteins that bind adjacent to the transcriptional initiation site of FIV promoter were also observed in FHV-1-infected CRFK cells, suggesting that the FHV-1-induced-proteins affects the transcription of FIV through the AP-1, ATF and lender sequences.

Original languageEnglish (US)
Pages (from-to)203-212
Number of pages10
JournalVirus Research
Volume51
Issue number2
DOIs
StatePublished - Oct 1 1997

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Keywords

  • AP-1
  • FHV-1
  • FIV
  • Forskolin
  • LBP-1
  • TPA

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

Cite this

Ikeda, Y., Kawaguchi, Y., Inoshima, Y., Kohmoto, M., Shimojima, M., Inada, G., Sato, E., Kai, C., Miyazawa, T., & Mikami, T. (1997). The effects of treatment with chemical agents or infection with feline viruses on protein-binding properties of the feline immunodeficiency virus long terminal repeat. Virus Research, 51(2), 203-212. https://doi.org/10.1016/S0168-1702(97)00094-4