The effects of treatment with chemical agents or infection with feline viruses on protein-binding properties of the feline immunodeficiency virus long terminal repeat

Yasuhiro H Ikeda, Yasushi Kawaguchi, Yasuo Inoshima, Mariko Kohmoto, Masayuki Shimojima, Gouki Inada, Eiji Sato, Chieko Kai, Takayuki Miyazawa, Takeshi Mikami

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The effects of treatment with chemical agents or infection with feline viruses on protein-binding properties of the feline immunodeficiency virus (FIV) long terminal repeat (LTR) were examined by gel-mobility-shift assays using oligonucleotides designed to represent putative AP-1 or ATF motif from the FIV LTR. Infection with FIV led to less nuclear proteins binding to the AP-1 and ATF sites, suggesting that proteins binding to the sites were consumed or suppressed by FIV-replication in FIV-infected cells. Nuclear proteins that bind to the AP-1 or ATF site were examined by using extracts from Crandell feline kidney (CRFK) cells treated with TPA (a phorbol ester; a strong activator of protein kinase C) or forskolin (an inducer of cyclic-AMP), or infection with feline herpesvirus type 1 (FHV-1). Although TPA or forskolin treatment moderately increased the level of both proteins that bound to AP-1 and ATF sites, FHV-1 infection markedly changed the protein-binding patterns of the sites. Furthermore, FHV-1-induced proteins that bind adjacent to the transcriptional initiation site of FIV promoter were also observed in FHV-1-infected CRFK cells, suggesting that the FHV-1-induced-proteins affects the transcription of FIV through the AP-1, ATF and lender sequences.

Original languageEnglish (US)
Pages (from-to)203-212
Number of pages10
JournalVirus Research
Volume51
Issue number2
DOIs
StatePublished - Oct 1997
Externally publishedYes

Fingerprint

Feline Immunodeficiency Virus
Terminal Repeat Sequences
Felidae
Protein Binding
Transcription Factor AP-1
Viruses
Herpesviridae
Infection
Colforsin
Nuclear Proteins
Binding Sites
Kidney
Herpesviridae Infections
Proteins
Electrophoretic Mobility Shift Assay
Phorbol Esters
Virus Replication
Oligonucleotides
Cyclic AMP
Protein Kinase C

Keywords

  • AP-1
  • FHV-1
  • FIV
  • Forskolin
  • LBP-1
  • TPA

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

The effects of treatment with chemical agents or infection with feline viruses on protein-binding properties of the feline immunodeficiency virus long terminal repeat. / Ikeda, Yasuhiro H; Kawaguchi, Yasushi; Inoshima, Yasuo; Kohmoto, Mariko; Shimojima, Masayuki; Inada, Gouki; Sato, Eiji; Kai, Chieko; Miyazawa, Takayuki; Mikami, Takeshi.

In: Virus Research, Vol. 51, No. 2, 10.1997, p. 203-212.

Research output: Contribution to journalArticle

Ikeda, YH, Kawaguchi, Y, Inoshima, Y, Kohmoto, M, Shimojima, M, Inada, G, Sato, E, Kai, C, Miyazawa, T & Mikami, T 1997, 'The effects of treatment with chemical agents or infection with feline viruses on protein-binding properties of the feline immunodeficiency virus long terminal repeat', Virus Research, vol. 51, no. 2, pp. 203-212. https://doi.org/10.1016/S0168-1702(97)00094-4
Ikeda, Yasuhiro H ; Kawaguchi, Yasushi ; Inoshima, Yasuo ; Kohmoto, Mariko ; Shimojima, Masayuki ; Inada, Gouki ; Sato, Eiji ; Kai, Chieko ; Miyazawa, Takayuki ; Mikami, Takeshi. / The effects of treatment with chemical agents or infection with feline viruses on protein-binding properties of the feline immunodeficiency virus long terminal repeat. In: Virus Research. 1997 ; Vol. 51, No. 2. pp. 203-212.
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