The effects of hyperglycaemia on isotopic measurement of glucose utilisation using [23H], [33H] and [614C] glucose in patients with Type 1 (insulin-dependent) diabetes mellitus

P. M. Bell, R. G. Firth, R. A. Rizza

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

To determine whether hyperglycaemia alters the accuracy with which [23H] and [33H]glucose reflect glucose turnover measured with [614C]glucose in patients with Type 1 (insulin-dependent) diabetes mellitus, glucose utilisation rates were measured during a simultaneous infusion of [23H], [33H] and [614C]glucose after maintenance of normoglycaemia overnight and when glucose concentrations were clamped at 5.3, 7.5 and 9.7 mmol/l while insulin and glucagon concentrations were held constant. Glucose utilisation rates determined with all three isotopes were comparable in the diabetic patients at all glucose concentrations studied. On the other hand, glucose utilisation rates in nondiabetic subjects determined with [614C]glucose were greater (p<0.01) than those determined with [33H]glucose and lower (p<0.04) than those determined with [23H]glucose during the 5.3, 7.5 and 9.7 mmol/l clamps. Nevertheless, glucose utilisation rates in the diabetic patients were lower (p<0.05) than those in the nondiabetic subjects for each glucose isotope. We conclude that hyperglycaemia does not alter the pattern of metabolism of [23H] or [33H]glucose in patients with Type 1 (insulin-dependent) diabetes mellitus.

Original languageEnglish (US)
Pages (from-to)365-371
Number of pages7
JournalDiabetologia
Volume30
Issue number6
DOIs
StatePublished - Jun 1987

Keywords

  • Type 1 (insulin-dependent) diabetes mellitus
  • [2H]glucose
  • [3H]glucose
  • [6C]glucose
  • glucose production
  • glucose utilisation
  • hyperglycaemia
  • insulin action

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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