The effects of halothane pretreatment on manganese influx induced by muscarinic stimulation of airway smooth muscle

David O. Warner, Keith A. Jones, Robert R. Lorenz

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We hypothesized that halothane inhibits contraction of canine airway smooth muscle in part by depleting sarcoplasmic reticulum (SR) calcium stores, which affects subsequent force and calcium influx. This hypothesis was tested by using the rate of quenching of fura-2 fluorescence by manganese (Mn2+) as an index of calcium influx. When added 10 min before submaximum muscarinic stimulation (with 0.3 μM acetylcholine [ACh]), halothane (0.60 ± 0.04 mM [mean ± sE]) reduced subsequent isometric force and intracellular calcium concentration ([Ca2+](i)) measured 10 min after contraction (to 55% ± 5% and 69% ± 4% of control, respectively). The Mn2+ influx measured concurrently was significantly increased by halothane (by 57% ± 22%). Depletion of SR calcium stores by ACh prior to contraction also increased Mn2+ influx (by 46% ± 6%) but did not affect developed force or increase [Ca2+](i) in response to submaximum muscarinic stimulation. Halothane did not affect [Ca2+](i) or Mn2+ influx when added prior to maximum stimulation with 100 μM ACh but significantly reduced developed force. These findings are consistent with the hypothesis that halothane-induced SR depletion prior to contraction stimulates subsequent calcium influx, but they further suggest that halothane-induced SR depletion itself does not contribute significantly to the reduction in contractility produced by halothane in the canine airway smooth muscle.

Original languageEnglish (US)
Pages (from-to)1366-1371
Number of pages6
JournalAnesthesia and analgesia
Volume84
Issue number6
DOIs
StatePublished - Jun 26 1997

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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