Abstract
The effects of growth differentiation factor-5 (GDF-5) and bone marrow stromal cells (BMSCs) on tendon healing were investigated under in vitro tissue culture conditions. BMSCs and GDF-5 placed in a collagen gel were interpositioned between the cut ends of dog flexor digitorum profundus tendons. The tendons were randomly assigned into four groups: 1) repaired tendon without gel; 2) repaired tendon with BMSC-seeded gel; 3) repaired tendon with GDF-5 gel without cells; and 4) repaired tendon with GDF-5 treated BMSC-seeded gel. At 2 and 4 weeks, the maximal strength of repaired tendons with GDF-5 treated BMSCs-seeded gel was significantly higher than in tendons without gel interposition. However, neither BMSCs nor GDF-5 alone significantly increased the maximal strength of healing tendons at 2 or 4 weeks. These results suggest that the combination of BMSCs and GDF-5 accelerates tendon healing, but either BMSCs or GDF-5 alone are not effective in this model.
Original language | English (US) |
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Pages (from-to) | 271-279 |
Number of pages | 9 |
Journal | Journal of Hand Surgery: European Volume |
Volume | 36 |
Issue number | 4 |
DOIs | |
State | Published - May 2011 |
Keywords
- Biomechanical testing
- Bone marrow stromal cell
- GDF-5
- In vitro
- Tendon healing
ASJC Scopus subject areas
- Surgery