The effects of bone marrow stromal cell transplants on tendon healing in vitro

Chunfeng D Zhao, Hsiao Feng Chieh, Karim Bakri, Jun Ikeda, Yu Long Sun, Steven Lawrence Moran, Kai N. An, Peter C Amadio

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The purpose of this study was to investigate the effect of bone marrow stromal cells (BMSCs) on tendon healing in a canine ex vivo model. Bone marrow was harvested and BMSCs were isolated and cultured according to established protocols. Cells were seeded into 0.5 mg/ml collagen gels and cultured for 24 h to allow gel contraction, and then implanted between the lacerated ends of repaired flexor digitorum profundus tendons. Tendons repaired with a gel patch alone and without a gel patch served as control groups. After 2 and 4 weeks in culture, the repaired tendons were evaluated for breaking strength and stiffness. Cell viability was assessed by labeling the cells with PKH26 red fluorescent cell linker. The maximal strength and stiffness of repaired tendons with the BMSC-seeded patch were significantly higher than the repaired tendons without a patch or with a patch without cells, at both 2 and 4 weeks (p < 0.05). Viable BMSC were present between the cut tendon ends at both 2 and 4 weeks. We conclude that BMSC-seeded gel patch transplantation has the potential to enhance flexor tendon healing, and we plan to investigate this effect in vivo.

Original languageEnglish (US)
Pages (from-to)1271-1275
Number of pages5
JournalMedical Engineering and Physics
Volume31
Issue number10
DOIs
StatePublished - Dec 2009

Fingerprint

Transplants
Tendons
Mesenchymal Stromal Cells
Bone
Gels
Stiffness
In Vitro Techniques
Collagen
Labeling
Canidae
Cell Survival
Transplantation
Bone Marrow
Cells
Control Groups

Keywords

  • Bone marrow stromal cell
  • Canine
  • In vitro
  • Tendon
  • Tendon healing

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering

Cite this

The effects of bone marrow stromal cell transplants on tendon healing in vitro. / Zhao, Chunfeng D; Chieh, Hsiao Feng; Bakri, Karim; Ikeda, Jun; Sun, Yu Long; Moran, Steven Lawrence; An, Kai N.; Amadio, Peter C.

In: Medical Engineering and Physics, Vol. 31, No. 10, 12.2009, p. 1271-1275.

Research output: Contribution to journalArticle

Zhao, Chunfeng D ; Chieh, Hsiao Feng ; Bakri, Karim ; Ikeda, Jun ; Sun, Yu Long ; Moran, Steven Lawrence ; An, Kai N. ; Amadio, Peter C. / The effects of bone marrow stromal cell transplants on tendon healing in vitro. In: Medical Engineering and Physics. 2009 ; Vol. 31, No. 10. pp. 1271-1275.
@article{103efd70c3374ec180dc4314d42eb3d0,
title = "The effects of bone marrow stromal cell transplants on tendon healing in vitro",
abstract = "The purpose of this study was to investigate the effect of bone marrow stromal cells (BMSCs) on tendon healing in a canine ex vivo model. Bone marrow was harvested and BMSCs were isolated and cultured according to established protocols. Cells were seeded into 0.5 mg/ml collagen gels and cultured for 24 h to allow gel contraction, and then implanted between the lacerated ends of repaired flexor digitorum profundus tendons. Tendons repaired with a gel patch alone and without a gel patch served as control groups. After 2 and 4 weeks in culture, the repaired tendons were evaluated for breaking strength and stiffness. Cell viability was assessed by labeling the cells with PKH26 red fluorescent cell linker. The maximal strength and stiffness of repaired tendons with the BMSC-seeded patch were significantly higher than the repaired tendons without a patch or with a patch without cells, at both 2 and 4 weeks (p < 0.05). Viable BMSC were present between the cut tendon ends at both 2 and 4 weeks. We conclude that BMSC-seeded gel patch transplantation has the potential to enhance flexor tendon healing, and we plan to investigate this effect in vivo.",
keywords = "Bone marrow stromal cell, Canine, In vitro, Tendon, Tendon healing",
author = "Zhao, {Chunfeng D} and Chieh, {Hsiao Feng} and Karim Bakri and Jun Ikeda and Sun, {Yu Long} and Moran, {Steven Lawrence} and An, {Kai N.} and Amadio, {Peter C}",
year = "2009",
month = "12",
doi = "10.1016/j.medengphy.2009.08.004",
language = "English (US)",
volume = "31",
pages = "1271--1275",
journal = "Medical Engineering and Physics",
issn = "1350-4533",
publisher = "Elsevier BV",
number = "10",

}

TY - JOUR

T1 - The effects of bone marrow stromal cell transplants on tendon healing in vitro

AU - Zhao, Chunfeng D

AU - Chieh, Hsiao Feng

AU - Bakri, Karim

AU - Ikeda, Jun

AU - Sun, Yu Long

AU - Moran, Steven Lawrence

AU - An, Kai N.

AU - Amadio, Peter C

PY - 2009/12

Y1 - 2009/12

N2 - The purpose of this study was to investigate the effect of bone marrow stromal cells (BMSCs) on tendon healing in a canine ex vivo model. Bone marrow was harvested and BMSCs were isolated and cultured according to established protocols. Cells were seeded into 0.5 mg/ml collagen gels and cultured for 24 h to allow gel contraction, and then implanted between the lacerated ends of repaired flexor digitorum profundus tendons. Tendons repaired with a gel patch alone and without a gel patch served as control groups. After 2 and 4 weeks in culture, the repaired tendons were evaluated for breaking strength and stiffness. Cell viability was assessed by labeling the cells with PKH26 red fluorescent cell linker. The maximal strength and stiffness of repaired tendons with the BMSC-seeded patch were significantly higher than the repaired tendons without a patch or with a patch without cells, at both 2 and 4 weeks (p < 0.05). Viable BMSC were present between the cut tendon ends at both 2 and 4 weeks. We conclude that BMSC-seeded gel patch transplantation has the potential to enhance flexor tendon healing, and we plan to investigate this effect in vivo.

AB - The purpose of this study was to investigate the effect of bone marrow stromal cells (BMSCs) on tendon healing in a canine ex vivo model. Bone marrow was harvested and BMSCs were isolated and cultured according to established protocols. Cells were seeded into 0.5 mg/ml collagen gels and cultured for 24 h to allow gel contraction, and then implanted between the lacerated ends of repaired flexor digitorum profundus tendons. Tendons repaired with a gel patch alone and without a gel patch served as control groups. After 2 and 4 weeks in culture, the repaired tendons were evaluated for breaking strength and stiffness. Cell viability was assessed by labeling the cells with PKH26 red fluorescent cell linker. The maximal strength and stiffness of repaired tendons with the BMSC-seeded patch were significantly higher than the repaired tendons without a patch or with a patch without cells, at both 2 and 4 weeks (p < 0.05). Viable BMSC were present between the cut tendon ends at both 2 and 4 weeks. We conclude that BMSC-seeded gel patch transplantation has the potential to enhance flexor tendon healing, and we plan to investigate this effect in vivo.

KW - Bone marrow stromal cell

KW - Canine

KW - In vitro

KW - Tendon

KW - Tendon healing

UR - http://www.scopus.com/inward/record.url?scp=70449535626&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70449535626&partnerID=8YFLogxK

U2 - 10.1016/j.medengphy.2009.08.004

DO - 10.1016/j.medengphy.2009.08.004

M3 - Article

VL - 31

SP - 1271

EP - 1275

JO - Medical Engineering and Physics

JF - Medical Engineering and Physics

SN - 1350-4533

IS - 10

ER -