The effect of time after shear injury on the subsynovial connective tissue and median nerve within the rabbit carpal tunnel

Matthias Vanhees, Takako Chikenji, Andrew R. Thoreson, Chunfeng D Zhao, James D. Schmelzer, Phillip Anson Low, Kai Nan An, Peter C Amadio

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: The most prominent nonneurological finding in the common compression neuropathy carpal tunnel syndrome (CTS) is fibrosis of the subsynovial connective tissue (SSCT). Recently, a rabbit model of CTS has been developed, based on the hypothesis that SSCT injury and subsequent fibrosis cause nerve compression. The purpose of this study was to evaluate the effects in this model at earlier and later time points than have heretofore been reported. Methods: Sixty rabbits were operated on and observed at two different time periods: 6 and 24 weeks. Nerve electrophysiology (EP), SSCT histology, and SSCT mechanical properties were assessed. Results: There was no significant difference in median motor nerve amplitude or latency at either time point. The total cell density in the SSCT was significantly higher at 6 and 24 weeks compared to controls. The mean size of the collagen fibrils in the SSCT was higher 6 and 24 weeks after surgery compared to controls. Both the ultimate load and the total energy absorption of the SSCT were significantly higher at 6 and 24 weeks compared to controls. Conclusions: In this model, there were signs of SSCT fibrosis and histology changes at 6 weeks, which persist after 24 weeks. Thus, this model leads to sustained SSCT fibrosis, which is one characteristic of human CTS. However, no significant EP changes were found at these two time points, which is in contrast to the findings reported previously for this model at 12 weeks. The significance of the differences in EP findings will be the subject of future studies.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalHand
Volume8
Issue number1
DOIs
StatePublished - 2013

Fingerprint

Median Nerve
Wrist
Connective Tissue
Rabbits
Wounds and Injuries
Carpal Tunnel Syndrome
Electrophysiology
Fibrosis
Histology
Collagen
Cell Count

Keywords

  • Carpal tunnel syndrome
  • Rabbit animal model
  • Shear injury
  • Subsynovial connective tissue

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

Cite this

The effect of time after shear injury on the subsynovial connective tissue and median nerve within the rabbit carpal tunnel. / Vanhees, Matthias; Chikenji, Takako; Thoreson, Andrew R.; Zhao, Chunfeng D; Schmelzer, James D.; Low, Phillip Anson; An, Kai Nan; Amadio, Peter C.

In: Hand, Vol. 8, No. 1, 2013, p. 54-59.

Research output: Contribution to journalArticle

Vanhees, Matthias ; Chikenji, Takako ; Thoreson, Andrew R. ; Zhao, Chunfeng D ; Schmelzer, James D. ; Low, Phillip Anson ; An, Kai Nan ; Amadio, Peter C. / The effect of time after shear injury on the subsynovial connective tissue and median nerve within the rabbit carpal tunnel. In: Hand. 2013 ; Vol. 8, No. 1. pp. 54-59.
@article{0505e5a8fa5844eaafa0520ecb132f8b,
title = "The effect of time after shear injury on the subsynovial connective tissue and median nerve within the rabbit carpal tunnel",
abstract = "Background: The most prominent nonneurological finding in the common compression neuropathy carpal tunnel syndrome (CTS) is fibrosis of the subsynovial connective tissue (SSCT). Recently, a rabbit model of CTS has been developed, based on the hypothesis that SSCT injury and subsequent fibrosis cause nerve compression. The purpose of this study was to evaluate the effects in this model at earlier and later time points than have heretofore been reported. Methods: Sixty rabbits were operated on and observed at two different time periods: 6 and 24 weeks. Nerve electrophysiology (EP), SSCT histology, and SSCT mechanical properties were assessed. Results: There was no significant difference in median motor nerve amplitude or latency at either time point. The total cell density in the SSCT was significantly higher at 6 and 24 weeks compared to controls. The mean size of the collagen fibrils in the SSCT was higher 6 and 24 weeks after surgery compared to controls. Both the ultimate load and the total energy absorption of the SSCT were significantly higher at 6 and 24 weeks compared to controls. Conclusions: In this model, there were signs of SSCT fibrosis and histology changes at 6 weeks, which persist after 24 weeks. Thus, this model leads to sustained SSCT fibrosis, which is one characteristic of human CTS. However, no significant EP changes were found at these two time points, which is in contrast to the findings reported previously for this model at 12 weeks. The significance of the differences in EP findings will be the subject of future studies.",
keywords = "Carpal tunnel syndrome, Rabbit animal model, Shear injury, Subsynovial connective tissue",
author = "Matthias Vanhees and Takako Chikenji and Thoreson, {Andrew R.} and Zhao, {Chunfeng D} and Schmelzer, {James D.} and Low, {Phillip Anson} and An, {Kai Nan} and Amadio, {Peter C}",
year = "2013",
doi = "10.1007/s11552-012-9469-2",
language = "English (US)",
volume = "8",
pages = "54--59",
journal = "Hand",
issn = "1558-9447",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - The effect of time after shear injury on the subsynovial connective tissue and median nerve within the rabbit carpal tunnel

AU - Vanhees, Matthias

AU - Chikenji, Takako

AU - Thoreson, Andrew R.

AU - Zhao, Chunfeng D

AU - Schmelzer, James D.

AU - Low, Phillip Anson

AU - An, Kai Nan

AU - Amadio, Peter C

PY - 2013

Y1 - 2013

N2 - Background: The most prominent nonneurological finding in the common compression neuropathy carpal tunnel syndrome (CTS) is fibrosis of the subsynovial connective tissue (SSCT). Recently, a rabbit model of CTS has been developed, based on the hypothesis that SSCT injury and subsequent fibrosis cause nerve compression. The purpose of this study was to evaluate the effects in this model at earlier and later time points than have heretofore been reported. Methods: Sixty rabbits were operated on and observed at two different time periods: 6 and 24 weeks. Nerve electrophysiology (EP), SSCT histology, and SSCT mechanical properties were assessed. Results: There was no significant difference in median motor nerve amplitude or latency at either time point. The total cell density in the SSCT was significantly higher at 6 and 24 weeks compared to controls. The mean size of the collagen fibrils in the SSCT was higher 6 and 24 weeks after surgery compared to controls. Both the ultimate load and the total energy absorption of the SSCT were significantly higher at 6 and 24 weeks compared to controls. Conclusions: In this model, there were signs of SSCT fibrosis and histology changes at 6 weeks, which persist after 24 weeks. Thus, this model leads to sustained SSCT fibrosis, which is one characteristic of human CTS. However, no significant EP changes were found at these two time points, which is in contrast to the findings reported previously for this model at 12 weeks. The significance of the differences in EP findings will be the subject of future studies.

AB - Background: The most prominent nonneurological finding in the common compression neuropathy carpal tunnel syndrome (CTS) is fibrosis of the subsynovial connective tissue (SSCT). Recently, a rabbit model of CTS has been developed, based on the hypothesis that SSCT injury and subsequent fibrosis cause nerve compression. The purpose of this study was to evaluate the effects in this model at earlier and later time points than have heretofore been reported. Methods: Sixty rabbits were operated on and observed at two different time periods: 6 and 24 weeks. Nerve electrophysiology (EP), SSCT histology, and SSCT mechanical properties were assessed. Results: There was no significant difference in median motor nerve amplitude or latency at either time point. The total cell density in the SSCT was significantly higher at 6 and 24 weeks compared to controls. The mean size of the collagen fibrils in the SSCT was higher 6 and 24 weeks after surgery compared to controls. Both the ultimate load and the total energy absorption of the SSCT were significantly higher at 6 and 24 weeks compared to controls. Conclusions: In this model, there were signs of SSCT fibrosis and histology changes at 6 weeks, which persist after 24 weeks. Thus, this model leads to sustained SSCT fibrosis, which is one characteristic of human CTS. However, no significant EP changes were found at these two time points, which is in contrast to the findings reported previously for this model at 12 weeks. The significance of the differences in EP findings will be the subject of future studies.

KW - Carpal tunnel syndrome

KW - Rabbit animal model

KW - Shear injury

KW - Subsynovial connective tissue

UR - http://www.scopus.com/inward/record.url?scp=84874018287&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874018287&partnerID=8YFLogxK

U2 - 10.1007/s11552-012-9469-2

DO - 10.1007/s11552-012-9469-2

M3 - Article

AN - SCOPUS:84874018287

VL - 8

SP - 54

EP - 59

JO - Hand

JF - Hand

SN - 1558-9447

IS - 1

ER -