The effect of postnatal growth retardation on abnormal neovascularization in the oxygen exposed neonatal rat

Jonathan M Holmes, Lisa A. Duffner

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Severe retinopathy of prematurity (ROP) occurs in the smallest and sickest of premature infants. We hypothesized that, in a rat model of oxygen induced retinopathy, abnormal neovascularization would occur more frequently in larger litters where the pups are subject to postnatal growth retardation. Four litters of newborn Sprague-Dawley rats were studied; rats were randomly mixed to form two large litters (n = 25 each) and two small litters (n = 10 each). All litters were exposed to 7 days cyclic hyperoxia and hypoxia followed by 5 days in room air. ADPase stained retinae were evaluated in a masked manner for the presence and severity of abnormal neovascularization. Fluorescein perfused retinae were digitized and the ratios of vascularized:total retinal area were calculated using computer assisted image analysis. As expected, final weight in the large litters was less than in the small litters (15.3 ± 3.8 g vs. 23.4 ± 2.1 g, p < 0.001). Neovascularization occurred in 53% of rats in the large litters vs. 15% in the small litters (p = 0.009). Rats with retinae demonstrating neovascularization were smaller than those without (16.2 ± 4.7 g vs. 19.6 ± 5.0 g, p = 0.016). The severity of neovascularization in clock h was inversely correlated with final weight (r(s) = -0.35, p = 0.01) and ratio of vascularized:total retina area (r(s) = -0.46, p < 0.001). Smaller rat pups raised in larger litters, with resultant growth retardation, develop more frequent and more severe abnormal retinal neovascularization. Our results correlate with clinical experience in the premature infant.

Original languageEnglish (US)
Pages (from-to)403-409
Number of pages7
JournalCurrent Eye Research
Volume15
Issue number4
StatePublished - 1996

Fingerprint

Retina
Oxygen
Growth
Premature Infants
Retinal Neovascularization
Apyrase
Weights and Measures
Retinopathy of Prematurity
Hyperoxia
Computer-Assisted Image Processing
Fluorescein
Sprague Dawley Rats
Air

Keywords

  • Growth retardation
  • Nutrition
  • Oxygen induced retinopathy
  • Rat
  • Retinal neovascularization
  • Retinopathy of prematurity

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

The effect of postnatal growth retardation on abnormal neovascularization in the oxygen exposed neonatal rat. / Holmes, Jonathan M; Duffner, Lisa A.

In: Current Eye Research, Vol. 15, No. 4, 1996, p. 403-409.

Research output: Contribution to journalArticle

@article{e55cc9d25f9e414fb58d0957c3281f9a,
title = "The effect of postnatal growth retardation on abnormal neovascularization in the oxygen exposed neonatal rat",
abstract = "Severe retinopathy of prematurity (ROP) occurs in the smallest and sickest of premature infants. We hypothesized that, in a rat model of oxygen induced retinopathy, abnormal neovascularization would occur more frequently in larger litters where the pups are subject to postnatal growth retardation. Four litters of newborn Sprague-Dawley rats were studied; rats were randomly mixed to form two large litters (n = 25 each) and two small litters (n = 10 each). All litters were exposed to 7 days cyclic hyperoxia and hypoxia followed by 5 days in room air. ADPase stained retinae were evaluated in a masked manner for the presence and severity of abnormal neovascularization. Fluorescein perfused retinae were digitized and the ratios of vascularized:total retinal area were calculated using computer assisted image analysis. As expected, final weight in the large litters was less than in the small litters (15.3 ± 3.8 g vs. 23.4 ± 2.1 g, p < 0.001). Neovascularization occurred in 53{\%} of rats in the large litters vs. 15{\%} in the small litters (p = 0.009). Rats with retinae demonstrating neovascularization were smaller than those without (16.2 ± 4.7 g vs. 19.6 ± 5.0 g, p = 0.016). The severity of neovascularization in clock h was inversely correlated with final weight (r(s) = -0.35, p = 0.01) and ratio of vascularized:total retina area (r(s) = -0.46, p < 0.001). Smaller rat pups raised in larger litters, with resultant growth retardation, develop more frequent and more severe abnormal retinal neovascularization. Our results correlate with clinical experience in the premature infant.",
keywords = "Growth retardation, Nutrition, Oxygen induced retinopathy, Rat, Retinal neovascularization, Retinopathy of prematurity",
author = "Holmes, {Jonathan M} and Duffner, {Lisa A.}",
year = "1996",
language = "English (US)",
volume = "15",
pages = "403--409",
journal = "Current Eye Research",
issn = "0271-3683",
publisher = "Informa Healthcare",
number = "4",

}

TY - JOUR

T1 - The effect of postnatal growth retardation on abnormal neovascularization in the oxygen exposed neonatal rat

AU - Holmes, Jonathan M

AU - Duffner, Lisa A.

PY - 1996

Y1 - 1996

N2 - Severe retinopathy of prematurity (ROP) occurs in the smallest and sickest of premature infants. We hypothesized that, in a rat model of oxygen induced retinopathy, abnormal neovascularization would occur more frequently in larger litters where the pups are subject to postnatal growth retardation. Four litters of newborn Sprague-Dawley rats were studied; rats were randomly mixed to form two large litters (n = 25 each) and two small litters (n = 10 each). All litters were exposed to 7 days cyclic hyperoxia and hypoxia followed by 5 days in room air. ADPase stained retinae were evaluated in a masked manner for the presence and severity of abnormal neovascularization. Fluorescein perfused retinae were digitized and the ratios of vascularized:total retinal area were calculated using computer assisted image analysis. As expected, final weight in the large litters was less than in the small litters (15.3 ± 3.8 g vs. 23.4 ± 2.1 g, p < 0.001). Neovascularization occurred in 53% of rats in the large litters vs. 15% in the small litters (p = 0.009). Rats with retinae demonstrating neovascularization were smaller than those without (16.2 ± 4.7 g vs. 19.6 ± 5.0 g, p = 0.016). The severity of neovascularization in clock h was inversely correlated with final weight (r(s) = -0.35, p = 0.01) and ratio of vascularized:total retina area (r(s) = -0.46, p < 0.001). Smaller rat pups raised in larger litters, with resultant growth retardation, develop more frequent and more severe abnormal retinal neovascularization. Our results correlate with clinical experience in the premature infant.

AB - Severe retinopathy of prematurity (ROP) occurs in the smallest and sickest of premature infants. We hypothesized that, in a rat model of oxygen induced retinopathy, abnormal neovascularization would occur more frequently in larger litters where the pups are subject to postnatal growth retardation. Four litters of newborn Sprague-Dawley rats were studied; rats were randomly mixed to form two large litters (n = 25 each) and two small litters (n = 10 each). All litters were exposed to 7 days cyclic hyperoxia and hypoxia followed by 5 days in room air. ADPase stained retinae were evaluated in a masked manner for the presence and severity of abnormal neovascularization. Fluorescein perfused retinae were digitized and the ratios of vascularized:total retinal area were calculated using computer assisted image analysis. As expected, final weight in the large litters was less than in the small litters (15.3 ± 3.8 g vs. 23.4 ± 2.1 g, p < 0.001). Neovascularization occurred in 53% of rats in the large litters vs. 15% in the small litters (p = 0.009). Rats with retinae demonstrating neovascularization were smaller than those without (16.2 ± 4.7 g vs. 19.6 ± 5.0 g, p = 0.016). The severity of neovascularization in clock h was inversely correlated with final weight (r(s) = -0.35, p = 0.01) and ratio of vascularized:total retina area (r(s) = -0.46, p < 0.001). Smaller rat pups raised in larger litters, with resultant growth retardation, develop more frequent and more severe abnormal retinal neovascularization. Our results correlate with clinical experience in the premature infant.

KW - Growth retardation

KW - Nutrition

KW - Oxygen induced retinopathy

KW - Rat

KW - Retinal neovascularization

KW - Retinopathy of prematurity

UR - http://www.scopus.com/inward/record.url?scp=0029872962&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029872962&partnerID=8YFLogxK

M3 - Article

C2 - 8670740

AN - SCOPUS:0029872962

VL - 15

SP - 403

EP - 409

JO - Current Eye Research

JF - Current Eye Research

SN - 0271-3683

IS - 4

ER -