The effect of oral dexamethasone on the circadian rhythm of aqueous humor flow in humans

P. T. Sheridan, R. F. Brubaker, L. I. Larsson, E. S. Rettig, William Francis Young

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose. To determine if the circadian rhythm of plasma corticosteroid activity is necessary for the circadian rhythm of aqueous humor flow to occur in humans. Methods. Twenty normal volunteers were recruited for this randomized, double-masked, placebo-controlled study. Oral dexamethasone was given at a dose of 0.5 mg every 6 hours; this is equivalent to more than two times the normal 24-hour endogenous adrenal corticosteroid production. This dosage schedule will maintain a relatively constant level of corticosteroid action throughout a 24-hour period. Topical fluorescein and a scanning fluorophotometer were used to measure the rate of aqueous humor flow. Subjects were studied on two separate days. On one study day, the subject took 0.5 mg of dexamethasone every 6 hours; on the other study day, the same subject took a placebo every 6 hours. Results. During the morning, aqueous flow was 4.06 ± 0.70 μl/min (mean ± SD) in subjects taking dexamethasone and 3.82 ± 0.85 in subjects taking a placebo. This 6% higher dexamethasone flow was not significant (P = 0.10). During the afternoon, aqueous flow was 3.83 ± 0.78 in subjects taking dexamethasone and 3.52 ± 0.77 in subjects taking a placebo. This 9% higher dexamethasone flow was statistically significant (P = 0.02). The nighttime aqueous flow was 1.38 ± 0.45 in subjects taking dexamethasone and 1.43 ± 0.34 in subjects taking the placebo. There was not a significant difference between placebo and dexamethasone during the night (P = 0.40). On each day, intraocular pressure was measured at 8:00 AM, 4:00 PM, and 6:00 AM. When comparing dexamethasone to placebo, no significant difference was observed in any of the intraocular pressures. Conclusions. The study is interpreted as showing that the reduction of aqueous humor flow during sleep can occur in the absence of a comparable fall in plasma corticosteroid activity.

Original languageEnglish (US)
Pages (from-to)1150-1156
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume35
Issue number3
StatePublished - 1994

Fingerprint

Aqueous Humor
Circadian Rhythm
Dexamethasone
Placebos
Adrenal Cortex Hormones
Intraocular Pressure
Fluorescein
Appointments and Schedules
Healthy Volunteers
Sleep

Keywords

  • aqueous humor flow
  • circadian rhythm
  • corticosteroids
  • human eye
  • steroid

ASJC Scopus subject areas

  • Ophthalmology

Cite this

The effect of oral dexamethasone on the circadian rhythm of aqueous humor flow in humans. / Sheridan, P. T.; Brubaker, R. F.; Larsson, L. I.; Rettig, E. S.; Young, William Francis.

In: Investigative Ophthalmology and Visual Science, Vol. 35, No. 3, 1994, p. 1150-1156.

Research output: Contribution to journalArticle

Sheridan, P. T. ; Brubaker, R. F. ; Larsson, L. I. ; Rettig, E. S. ; Young, William Francis. / The effect of oral dexamethasone on the circadian rhythm of aqueous humor flow in humans. In: Investigative Ophthalmology and Visual Science. 1994 ; Vol. 35, No. 3. pp. 1150-1156.
@article{f9993b4936f747b88d8c25e7c3f74b5f,
title = "The effect of oral dexamethasone on the circadian rhythm of aqueous humor flow in humans",
abstract = "Purpose. To determine if the circadian rhythm of plasma corticosteroid activity is necessary for the circadian rhythm of aqueous humor flow to occur in humans. Methods. Twenty normal volunteers were recruited for this randomized, double-masked, placebo-controlled study. Oral dexamethasone was given at a dose of 0.5 mg every 6 hours; this is equivalent to more than two times the normal 24-hour endogenous adrenal corticosteroid production. This dosage schedule will maintain a relatively constant level of corticosteroid action throughout a 24-hour period. Topical fluorescein and a scanning fluorophotometer were used to measure the rate of aqueous humor flow. Subjects were studied on two separate days. On one study day, the subject took 0.5 mg of dexamethasone every 6 hours; on the other study day, the same subject took a placebo every 6 hours. Results. During the morning, aqueous flow was 4.06 ± 0.70 μl/min (mean ± SD) in subjects taking dexamethasone and 3.82 ± 0.85 in subjects taking a placebo. This 6{\%} higher dexamethasone flow was not significant (P = 0.10). During the afternoon, aqueous flow was 3.83 ± 0.78 in subjects taking dexamethasone and 3.52 ± 0.77 in subjects taking a placebo. This 9{\%} higher dexamethasone flow was statistically significant (P = 0.02). The nighttime aqueous flow was 1.38 ± 0.45 in subjects taking dexamethasone and 1.43 ± 0.34 in subjects taking the placebo. There was not a significant difference between placebo and dexamethasone during the night (P = 0.40). On each day, intraocular pressure was measured at 8:00 AM, 4:00 PM, and 6:00 AM. When comparing dexamethasone to placebo, no significant difference was observed in any of the intraocular pressures. Conclusions. The study is interpreted as showing that the reduction of aqueous humor flow during sleep can occur in the absence of a comparable fall in plasma corticosteroid activity.",
keywords = "aqueous humor flow, circadian rhythm, corticosteroids, human eye, steroid",
author = "Sheridan, {P. T.} and Brubaker, {R. F.} and Larsson, {L. I.} and Rettig, {E. S.} and Young, {William Francis}",
year = "1994",
language = "English (US)",
volume = "35",
pages = "1150--1156",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "3",

}

TY - JOUR

T1 - The effect of oral dexamethasone on the circadian rhythm of aqueous humor flow in humans

AU - Sheridan, P. T.

AU - Brubaker, R. F.

AU - Larsson, L. I.

AU - Rettig, E. S.

AU - Young, William Francis

PY - 1994

Y1 - 1994

N2 - Purpose. To determine if the circadian rhythm of plasma corticosteroid activity is necessary for the circadian rhythm of aqueous humor flow to occur in humans. Methods. Twenty normal volunteers were recruited for this randomized, double-masked, placebo-controlled study. Oral dexamethasone was given at a dose of 0.5 mg every 6 hours; this is equivalent to more than two times the normal 24-hour endogenous adrenal corticosteroid production. This dosage schedule will maintain a relatively constant level of corticosteroid action throughout a 24-hour period. Topical fluorescein and a scanning fluorophotometer were used to measure the rate of aqueous humor flow. Subjects were studied on two separate days. On one study day, the subject took 0.5 mg of dexamethasone every 6 hours; on the other study day, the same subject took a placebo every 6 hours. Results. During the morning, aqueous flow was 4.06 ± 0.70 μl/min (mean ± SD) in subjects taking dexamethasone and 3.82 ± 0.85 in subjects taking a placebo. This 6% higher dexamethasone flow was not significant (P = 0.10). During the afternoon, aqueous flow was 3.83 ± 0.78 in subjects taking dexamethasone and 3.52 ± 0.77 in subjects taking a placebo. This 9% higher dexamethasone flow was statistically significant (P = 0.02). The nighttime aqueous flow was 1.38 ± 0.45 in subjects taking dexamethasone and 1.43 ± 0.34 in subjects taking the placebo. There was not a significant difference between placebo and dexamethasone during the night (P = 0.40). On each day, intraocular pressure was measured at 8:00 AM, 4:00 PM, and 6:00 AM. When comparing dexamethasone to placebo, no significant difference was observed in any of the intraocular pressures. Conclusions. The study is interpreted as showing that the reduction of aqueous humor flow during sleep can occur in the absence of a comparable fall in plasma corticosteroid activity.

AB - Purpose. To determine if the circadian rhythm of plasma corticosteroid activity is necessary for the circadian rhythm of aqueous humor flow to occur in humans. Methods. Twenty normal volunteers were recruited for this randomized, double-masked, placebo-controlled study. Oral dexamethasone was given at a dose of 0.5 mg every 6 hours; this is equivalent to more than two times the normal 24-hour endogenous adrenal corticosteroid production. This dosage schedule will maintain a relatively constant level of corticosteroid action throughout a 24-hour period. Topical fluorescein and a scanning fluorophotometer were used to measure the rate of aqueous humor flow. Subjects were studied on two separate days. On one study day, the subject took 0.5 mg of dexamethasone every 6 hours; on the other study day, the same subject took a placebo every 6 hours. Results. During the morning, aqueous flow was 4.06 ± 0.70 μl/min (mean ± SD) in subjects taking dexamethasone and 3.82 ± 0.85 in subjects taking a placebo. This 6% higher dexamethasone flow was not significant (P = 0.10). During the afternoon, aqueous flow was 3.83 ± 0.78 in subjects taking dexamethasone and 3.52 ± 0.77 in subjects taking a placebo. This 9% higher dexamethasone flow was statistically significant (P = 0.02). The nighttime aqueous flow was 1.38 ± 0.45 in subjects taking dexamethasone and 1.43 ± 0.34 in subjects taking the placebo. There was not a significant difference between placebo and dexamethasone during the night (P = 0.40). On each day, intraocular pressure was measured at 8:00 AM, 4:00 PM, and 6:00 AM. When comparing dexamethasone to placebo, no significant difference was observed in any of the intraocular pressures. Conclusions. The study is interpreted as showing that the reduction of aqueous humor flow during sleep can occur in the absence of a comparable fall in plasma corticosteroid activity.

KW - aqueous humor flow

KW - circadian rhythm

KW - corticosteroids

KW - human eye

KW - steroid

UR - http://www.scopus.com/inward/record.url?scp=0028359260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028359260&partnerID=8YFLogxK

M3 - Article

C2 - 8125726

AN - SCOPUS:0028359260

VL - 35

SP - 1150

EP - 1156

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 3

ER -