The effect of nasal polyp epithelial cells on eosinophil activation

Seung Heon Shin, Sang Heon Lee, Hyo Soon Jeong, Hirohito Kita

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Objectives/Hypothesis: Eosinophil infiltration into an inflammatory site is a characteristic histological finding in patients with chronic rhinosinusitis and nasal polyps. Most of the eosinophils in chronic rhinosinusitis are activated in the nasal cavity, but the exact activation mechanism of eosinophils is unknown. The study was designed to investigate the effect of human nasal epithelial cells on the activation of eosinophils. Study Design: Peripheral blood eosinophils were isolated from healthy volunteers and incubated in human nasal polyp epithelial cell conditioned media (HPECM). Superoxide production and eosinophil-derived neurotoxin were measured to determine eosinophils activation. HPECMs were assayed by ELISAs for interleukin-8 (IL-8), granulocyte-macrophage colony stimulating factor (GM-CSF), eotaxin, and regulated on activation normal T expressed and secreted (RANTES). To identify the chemical mediators involved in the activation of eosinophils. Results: HPECM (n = 7) contained 31.48 ng/mL interleukin-8, 533.43 pg/mL GM-CSF, 5.90 pg/mL eotaxin, and 11.06 pg/mL RANTES. Eosinophils were activated by HPECM and inhibited only by anti-GM-CSF antibody, not by the other chemical mediators. Conclusion: The results suggest that eosinophils in nasal secretions are activated by GM-CSF, which is produced by nasal epithelial cells.

Original languageEnglish (US)
Pages (from-to)1374-1377
Number of pages4
JournalLaryngoscope
Volume113
Issue number8
DOIs
StatePublished - Aug 1 2003

Keywords

  • Cytokine
  • Eosinophil
  • Epithelial cell
  • Nasal polyp

ASJC Scopus subject areas

  • Otorhinolaryngology

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