The effect of laminin peptide gradient in enzymatically cross-linked collagen scaffolds on neurite growth

Li Yao, Gopinath Damodaran, Natalia Nikolskaya, Adrienne M. Gorman, Anthony Windebank, Abhay Pandit

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Guided neurite growth is critical in both peripheral nervous system and central nervous system nerve regeneration. Scaffolds that provide structural and guidance cues for neuronal cells have a potential role in neural regeneration application. Type I collagen is suitable to be processed as an engineered scaffold for nerve regeneration because of its biological and structural properties. A few previous studies have shown that cross-linking of collagen scaffolds with microbial transglutaminase improves the mechanical strength and degradation properties of the scaffolds. It was shown that laminin 5 can regulate neurite outgrowth and extension. A motif (PPFLMLLKGSTR) in the human laminin 5 a3 chain is crucial for both integrin a3b1 receptor binding and cell adhesion. In the present work, we studied the guidance effect of a laminin peptide (PPFLMLLKGSTR) gradient in collagen and cross-linked collagen scaffolds on neurite growth. Neurites of rat pheochromocytoma (PC12) cells showed a preferential growth toward the high laminin concentration level on the collagen scaffold, while the incorporation of laminin peptide in the scaffold did not influence neurite length of PC12 cells.

Original languageEnglish (US)
Pages (from-to)484-492
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume92
Issue number2
DOIs
StatePublished - Feb 1 2010

Keywords

  • Collagen
  • Gradient
  • Laminin peptide
  • Microbial transglutaminase
  • Neurite

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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