The effect of intraluminal contact mediated guidance signals on axonal mismatch during peripheral nerve repair

William T. Daly, Li Yao, Mohammad T. Abu-rub, Claire O'Connell, Dimitrios I. Zeugolis, Anthony J. Windebank, Abhay S. Pandit

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

The current microsurgical gold standard for repairing long gap nerve injuries is the autograft. Autograft provides a protective environment for repair and a natural internal architecture, which is essential for regeneration. Current clinically approved hollow nerve guidance conduits allow provision of this protective environment; however they fail to provide an essential internal architecture to the regenerating nerve. In the present study both structured and unstructured intraluminal collagen fibres are investigated to assess their ability to enhance conduit mediated nerve repair. This study presents a direct comparison of both structured and unstructured fibres in vivo. The addition of intraluminal guidance structures was shown to significantly decrease axonal dispersion within the conduit and reduced axonal mismatch of distal nerve targets (p < 0.05). The intraluminal fibres were shown to be successfully incorporated into the host regenerative process, acting as a platform for Schwann cell migration and axonal regeneration. Ultimately the fibres were able to provide a platform for nerve regeneration in a long term regeneration study (16 weeks) and facilitated increased guidance of regenerating axons towards their distal nerve targets.

Original languageEnglish (US)
Pages (from-to)6660-6671
Number of pages12
JournalBiomaterials
Volume33
Issue number28
DOIs
StatePublished - Oct 2012

Keywords

  • Biomaterials
  • In vivo
  • Nerve guidance conduit
  • Nerve guide
  • Peripheral nerve
  • Repair

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Fingerprint Dive into the research topics of 'The effect of intraluminal contact mediated guidance signals on axonal mismatch during peripheral nerve repair'. Together they form a unique fingerprint.

Cite this