The effect of granulocyte colony stimulating factor on regional and global myocardial function in the porcine infarct model

Steve S. Lee, Tasneem Z. Naqvi, James Forrester, Russ Cattley, Atman Shah, Malka Frantzen, Takashi Miyamoto, Stephen Kaufman, Matthew J. Price, Michael Lill, Raj R. Makkar

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Background: Stem cell therapy has been shown to attenuate the reduction of left ventricular function following myocardial infarction. Most studies have utilized either a direct injection or intra-coronary infusion of cells, but cytokine mobilization of stem cells in the murine model of acute myocardial infarction has been reported to induce similar improvement in cardiac function. Methods: An antero-apical infarction was induced in swine by balloon occlusion, followed by the daily administration of granulocyte colony stimulating factor (G-CSF) or placebo for 5 days. We used left ventricular angiograms and 2D echocardiograms to assess global function, and 3D echocardiograms to assess regional function prior to infarction, immediately following infarction, and at 8 weeks. Histologic evaluation was performed after sacrifice at 8 weeks. Results: There was no significant difference in early or late post-infarction left ventricular ejection fraction or in myocardial histology between the two groups. Following G-CSF therapy, however, 3D echocardiography demonstrated that the regional ejection fractions of the infarcted segments showed a 50.3% improvement in the G-CSF pigs compared to a 7.4% deterioration in the untreated pigs (p = 0.005). Conclusions: Global left ventricular ejection fraction remained unchanged, and there is no histologic evidence for infarct attenuation following G-CSF infusion in the porcine infarct-reperfusion model. There was recovery of regional function in the infarcted segment in the G-CSF pigs. These data suggest that bone marrow mobilization in larger species has limited potential as a therapy designed to replace infarcted myocardium or to improve overall cardiac function, although further studies are needed to examine regional effect in the infarct area.

Original languageEnglish (US)
Pages (from-to)225-230
Number of pages6
JournalInternational Journal of Cardiology
Volume116
Issue number2
DOIs
StatePublished - Mar 20 2007

Keywords

  • G-CSF
  • Left ventricular ejection fraction
  • Myocardial infarction
  • Stem cells

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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