THE EFFECT OF FREEZING ON BIOMECHANICAL PROPERTIES OF THE CARPAL TUNNEL SUBSYNOVIAL CONNECTIVE TISSUE

Anika Filius, Andrew Thoreson, Abhishek Dharan, Kristin Mara, Kai Nan An, Chunfeng D Zhao, Peter C Amadio

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Biomechanical evaluation of the subsynovial connective tissue (SSCT) provides insight into the causes of carpal tunnel syndrome. Studies of carpal tunnel mechanics have been performed using fresh-frozen cadaver tissue. Freezing can affect mechanical properties of some tissues, but its effect on SSCT is unknown. A total of 16 rabbit paws were harvested from eight New Zealand rabbits and subjected to mechanical testing consisting of three repeated tendon excursions in sets of three different excursion magnitudes. One paw from each animal was unfrozen. The contralateral paw was frozen and thawed before testing. Force, energy and stiffness of the first cycle of each set were evaluated, as were ratios of the second to first cycle for each parameter. Two-factor ANOVA with repeated measures over both factors was performed. No significant interactions between factors were found. There were no significant differences between fresh and frozen paws for any parameters, though there were significant differences between excursion amplitudes. The damage threshold was not different between fresh and frozen paws. This study demonstrated that freezing rabbit subsynovial connective tissue does not significantly change its mechanical properties. The same may be true for human cadaver tissues. Results of cadaver mechanical testing may not be influenced by this preservation technique.

Original languageEnglish (US)
JournalJournal of Musculoskeletal Research
DOIs
StateAccepted/In press - 2016

Fingerprint

Wrist
Cadaver
Connective Tissue
Freezing
Rabbits
Carpal Tunnel Syndrome
Mechanics
Tendons
Analysis of Variance

Keywords

  • Cadaver
  • Carpal tunnel syndrome
  • Fibrosis
  • Synovium
  • Wrist

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

THE EFFECT OF FREEZING ON BIOMECHANICAL PROPERTIES OF THE CARPAL TUNNEL SUBSYNOVIAL CONNECTIVE TISSUE. / Filius, Anika; Thoreson, Andrew; Dharan, Abhishek; Mara, Kristin; An, Kai Nan; Zhao, Chunfeng D; Amadio, Peter C.

In: Journal of Musculoskeletal Research, 2016.

Research output: Contribution to journalArticle

@article{82d065ce9d0846c6bc13a8c5d972fe22,
title = "THE EFFECT OF FREEZING ON BIOMECHANICAL PROPERTIES OF THE CARPAL TUNNEL SUBSYNOVIAL CONNECTIVE TISSUE",
abstract = "Biomechanical evaluation of the subsynovial connective tissue (SSCT) provides insight into the causes of carpal tunnel syndrome. Studies of carpal tunnel mechanics have been performed using fresh-frozen cadaver tissue. Freezing can affect mechanical properties of some tissues, but its effect on SSCT is unknown. A total of 16 rabbit paws were harvested from eight New Zealand rabbits and subjected to mechanical testing consisting of three repeated tendon excursions in sets of three different excursion magnitudes. One paw from each animal was unfrozen. The contralateral paw was frozen and thawed before testing. Force, energy and stiffness of the first cycle of each set were evaluated, as were ratios of the second to first cycle for each parameter. Two-factor ANOVA with repeated measures over both factors was performed. No significant interactions between factors were found. There were no significant differences between fresh and frozen paws for any parameters, though there were significant differences between excursion amplitudes. The damage threshold was not different between fresh and frozen paws. This study demonstrated that freezing rabbit subsynovial connective tissue does not significantly change its mechanical properties. The same may be true for human cadaver tissues. Results of cadaver mechanical testing may not be influenced by this preservation technique.",
keywords = "Cadaver, Carpal tunnel syndrome, Fibrosis, Synovium, Wrist",
author = "Anika Filius and Andrew Thoreson and Abhishek Dharan and Kristin Mara and An, {Kai Nan} and Zhao, {Chunfeng D} and Amadio, {Peter C}",
year = "2016",
doi = "10.1142/S0218957715500190",
language = "English (US)",
journal = "Journal of Musculoskeletal Research",
issn = "0218-9577",
publisher = "World Scientific Publishing Co. Pte Ltd",

}

TY - JOUR

T1 - THE EFFECT OF FREEZING ON BIOMECHANICAL PROPERTIES OF THE CARPAL TUNNEL SUBSYNOVIAL CONNECTIVE TISSUE

AU - Filius, Anika

AU - Thoreson, Andrew

AU - Dharan, Abhishek

AU - Mara, Kristin

AU - An, Kai Nan

AU - Zhao, Chunfeng D

AU - Amadio, Peter C

PY - 2016

Y1 - 2016

N2 - Biomechanical evaluation of the subsynovial connective tissue (SSCT) provides insight into the causes of carpal tunnel syndrome. Studies of carpal tunnel mechanics have been performed using fresh-frozen cadaver tissue. Freezing can affect mechanical properties of some tissues, but its effect on SSCT is unknown. A total of 16 rabbit paws were harvested from eight New Zealand rabbits and subjected to mechanical testing consisting of three repeated tendon excursions in sets of three different excursion magnitudes. One paw from each animal was unfrozen. The contralateral paw was frozen and thawed before testing. Force, energy and stiffness of the first cycle of each set were evaluated, as were ratios of the second to first cycle for each parameter. Two-factor ANOVA with repeated measures over both factors was performed. No significant interactions between factors were found. There were no significant differences between fresh and frozen paws for any parameters, though there were significant differences between excursion amplitudes. The damage threshold was not different between fresh and frozen paws. This study demonstrated that freezing rabbit subsynovial connective tissue does not significantly change its mechanical properties. The same may be true for human cadaver tissues. Results of cadaver mechanical testing may not be influenced by this preservation technique.

AB - Biomechanical evaluation of the subsynovial connective tissue (SSCT) provides insight into the causes of carpal tunnel syndrome. Studies of carpal tunnel mechanics have been performed using fresh-frozen cadaver tissue. Freezing can affect mechanical properties of some tissues, but its effect on SSCT is unknown. A total of 16 rabbit paws were harvested from eight New Zealand rabbits and subjected to mechanical testing consisting of three repeated tendon excursions in sets of three different excursion magnitudes. One paw from each animal was unfrozen. The contralateral paw was frozen and thawed before testing. Force, energy and stiffness of the first cycle of each set were evaluated, as were ratios of the second to first cycle for each parameter. Two-factor ANOVA with repeated measures over both factors was performed. No significant interactions between factors were found. There were no significant differences between fresh and frozen paws for any parameters, though there were significant differences between excursion amplitudes. The damage threshold was not different between fresh and frozen paws. This study demonstrated that freezing rabbit subsynovial connective tissue does not significantly change its mechanical properties. The same may be true for human cadaver tissues. Results of cadaver mechanical testing may not be influenced by this preservation technique.

KW - Cadaver

KW - Carpal tunnel syndrome

KW - Fibrosis

KW - Synovium

KW - Wrist

UR - http://www.scopus.com/inward/record.url?scp=84964033558&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964033558&partnerID=8YFLogxK

U2 - 10.1142/S0218957715500190

DO - 10.1142/S0218957715500190

M3 - Article

AN - SCOPUS:84964033558

JO - Journal of Musculoskeletal Research

JF - Journal of Musculoskeletal Research

SN - 0218-9577

ER -