The effect of ethanol on the passive Ca permeability of human red cell ghosts measured by means of arsenazo III

Douglas R. Yingst, Petra Marcela Casey, Paul Kilgore

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Ethanol in the range of 0.76-2.40 M caused an immediate increase in the Ca permeability of the plasma membrane of resealed human red blood cell ghosts in which intracellular free Ca could be continuously monitored by means of the Ca chromophore arsenazo III. At a given concentration of ethanol, the Ca permeability increased markedly a few minutes following the mixing of the ghosts and the ethanol, and continued to increase over at least the next 30 min. Preincubating the ghosts in ethanol for 15, 60 and 120 min before measuring the rate of free Ca accumulation, progressively increased the effect of a given concentration of ethanol. These results indicate that the effect of a given concentration of ethanol is a complex function of concentration and exposure time. The effects of ethanol in this concentration range were completely reversible. The resealed ghosts used in these experiments were depleted of ATP to avoid interference from the Ca pump and all experiments were carried out with 150 mM KCl on both sides of the membrane to minimize changes in either the volume or membrane potential associated with activation of the Ca-dependent K channel.

Original languageEnglish (US)
Pages (from-to)277-281
Number of pages5
JournalBBA - Biomembranes
Volume813
Issue number2
DOIs
StatePublished - Mar 14 1985
Externally publishedYes

Fingerprint

Arsenazo III
Erythrocyte Membrane
Permeability
Ethanol
Cells
Membranes
Cell membranes
Chromophores
Membrane Potentials
Blood
Adenosine Triphosphate
Erythrocytes
Experiments
Chemical activation
Cell Membrane
Pumps

Keywords

  • (Erythrocyte membrane)
  • Arsenazo III
  • Ca transport
  • Ethanol
  • Passive transport

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

The effect of ethanol on the passive Ca permeability of human red cell ghosts measured by means of arsenazo III. / Yingst, Douglas R.; Casey, Petra Marcela; Kilgore, Paul.

In: BBA - Biomembranes, Vol. 813, No. 2, 14.03.1985, p. 277-281.

Research output: Contribution to journalArticle

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N2 - Ethanol in the range of 0.76-2.40 M caused an immediate increase in the Ca permeability of the plasma membrane of resealed human red blood cell ghosts in which intracellular free Ca could be continuously monitored by means of the Ca chromophore arsenazo III. At a given concentration of ethanol, the Ca permeability increased markedly a few minutes following the mixing of the ghosts and the ethanol, and continued to increase over at least the next 30 min. Preincubating the ghosts in ethanol for 15, 60 and 120 min before measuring the rate of free Ca accumulation, progressively increased the effect of a given concentration of ethanol. These results indicate that the effect of a given concentration of ethanol is a complex function of concentration and exposure time. The effects of ethanol in this concentration range were completely reversible. The resealed ghosts used in these experiments were depleted of ATP to avoid interference from the Ca pump and all experiments were carried out with 150 mM KCl on both sides of the membrane to minimize changes in either the volume or membrane potential associated with activation of the Ca-dependent K channel.

AB - Ethanol in the range of 0.76-2.40 M caused an immediate increase in the Ca permeability of the plasma membrane of resealed human red blood cell ghosts in which intracellular free Ca could be continuously monitored by means of the Ca chromophore arsenazo III. At a given concentration of ethanol, the Ca permeability increased markedly a few minutes following the mixing of the ghosts and the ethanol, and continued to increase over at least the next 30 min. Preincubating the ghosts in ethanol for 15, 60 and 120 min before measuring the rate of free Ca accumulation, progressively increased the effect of a given concentration of ethanol. These results indicate that the effect of a given concentration of ethanol is a complex function of concentration and exposure time. The effects of ethanol in this concentration range were completely reversible. The resealed ghosts used in these experiments were depleted of ATP to avoid interference from the Ca pump and all experiments were carried out with 150 mM KCl on both sides of the membrane to minimize changes in either the volume or membrane potential associated with activation of the Ca-dependent K channel.

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