The different effects of two thymic stromal cells on programmed cell death of thymocytes cultured in vitro

Haidong M Dong, Xuewen Pang, Weifeng Chen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We investigated the regulation of thymic stromal cells on the process of programmed cell death of thymocytes. By using DNA electrophoreses and propidium iodide-DNA staining flow cytometry assay, we investigated the effects of mouse thymic stromal cells (MTSC) on the process of programmed cell death of cultured thymocytes. The progressive DNA fragmentation and higher percentage of apoptotic cells hypodiploid DNA were observed in thymocytes at subsequent time point. When the thymocytes were cocultured with mouse thymic epithelial cell line (MTEC1), a sharp decrease of DNA fragmentation was observed in thymocytes at different culture time, while the mouse thymic dendritic cell line (MTSC4) enhanced the DNA fragmentation of thymocytes at 12 to 18 h-coculture time. FACS assay quantitatively confirmed these observation. MTEC1, but not MTSC4 enhanced the survival rate of thymocytes in vitro. By histochemistry DNA in situ staining method, we observed the presence of apoptotic cells around the two thymic stromal cells. These results suggest that thymic stromal cells may regulate the process of thymocytes during the negative selection in thymus through different mechanisms in which providing survival signals and engulfing apoptotic cells may be included.

Original languageEnglish (US)
Pages (from-to)284-288
Number of pages5
JournalChinese Journal of Microbiology and Immunology
Volume16
Issue number4
StatePublished - 1996
Externally publishedYes

Fingerprint

Thymocytes
Stromal Cells
Cell Death
DNA Fragmentation
DNA
Staining and Labeling
Cell Line
In Vitro Techniques
Propidium
Coculture Techniques
Dendritic Cells
Thymus Gland
Flow Cytometry
Epithelial Cells
Observation

Keywords

  • Apoptosis
  • Thymic stromal cell
  • Thymocyte

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)

Cite this

The different effects of two thymic stromal cells on programmed cell death of thymocytes cultured in vitro. / Dong, Haidong M; Pang, Xuewen; Chen, Weifeng.

In: Chinese Journal of Microbiology and Immunology, Vol. 16, No. 4, 1996, p. 284-288.

Research output: Contribution to journalArticle

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