The diagnostic utility of combination of HMGA2 and IMP3 qRT-PCR testing in thyroid neoplasms

Long Jin, Ricardo V. Lloyd, Michael R. Henry, Lori A. Erickson, Thomas J. Sebo, Kandelaria M. Rumilla, Jun Zhang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The diagnosis of malignant thyroid tumors in some cytologic and histologic specimens remains challenging. Highmobility group A2 (HMGA2) expression and insulin-like growth factor II mRNA-binding protein-3 (IMP3) expression were evaluated by relative quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The aim of this study was to evaluate whether the combination of HMGA2 and IMP3 qRTPCR was diagnostically useful in differentiating benign from malignant thyroid neoplasms. Fine-needle aspiration (FNA) specimens from 120 patients including 56 benign lesions and 64 carcinomas were used. The available 80 corresponding formalinfixed paraffin-embedded (FFPE) thyroid tissues from 66 patients were also included in this study. HMGA2 and IMP3 expression levels were detected by qRT-PCR and reported as relative fold change after normalizing with a calibrator. The diagnostic utilities of HMGA2 and IMP3 qRT-PCR tests were evaluated individually and in combination. In FNA specimens, HMGA2 and IMP3 expression was consistently higher in thyroid malignancies compared with benign lesions in all subgroups except in Hü rthle cell tumors. After exclusion of Hü rthle cell tumors, the sensitivity was 90.2% for HMGA2, 88.2% for IMP3, and 98% for HMGA2+IMP3; the specificity was 97.1% for HMGA2, 79.4% for IMP3, and 79.4% for HMGA+IMP3. qRT-PCR data showed similar results in FFPE tissues: the sensitivity was 84.2% for HMGA2, 85.7% for IMP3, and 94.7% for HMGA2+IMP3; the specificity was 96.9% for HMGA2, 91.2% for IMP3, and 90.6% for HMGA2+IMP3. qRT-PCR data were concordant between FNA and FFPE samples for HMGA2 (97.4%) and IMP3 (96.9%). The results indicate that HMGA2 qRT-PCR with high specificity may be a useful ancillary technique to assist in the classification of difficult thyroid specimens, excluding Hü rthle cell tumors. The HMGA2 and IMP3 qRTPCR combination model with increased sensitivity and negative predictive value (96.4%) may be useful in screening thyroid cytology specimens.

Original languageEnglish (US)
Pages (from-to)36-43
Number of pages8
JournalApplied Immunohistochemistry and Molecular Morphology
Volume23
Issue number1
StatePublished - Jan 14 2015

Fingerprint

Thyroid Neoplasms
varespladib methyl
Reverse Transcription
Carrier Proteins
Polymerase Chain Reaction
Messenger RNA
Thyroid Gland
Fine Needle Biopsy
Paraffin
Neoplasms
HMGA Proteins
Insulin-Like Growth Factor II
Cell Biology

Keywords

  • HMGA2
  • IMP3
  • Quantitative RT-PCR
  • Thyroid

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology
  • Histology

Cite this

Jin, L., Lloyd, R. V., Henry, M. R., Erickson, L. A., Sebo, T. J., Rumilla, K. M., & Zhang, J. (2015). The diagnostic utility of combination of HMGA2 and IMP3 qRT-PCR testing in thyroid neoplasms. Applied Immunohistochemistry and Molecular Morphology, 23(1), 36-43.

The diagnostic utility of combination of HMGA2 and IMP3 qRT-PCR testing in thyroid neoplasms. / Jin, Long; Lloyd, Ricardo V.; Henry, Michael R.; Erickson, Lori A.; Sebo, Thomas J.; Rumilla, Kandelaria M.; Zhang, Jun.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 23, No. 1, 14.01.2015, p. 36-43.

Research output: Contribution to journalArticle

Jin, L, Lloyd, RV, Henry, MR, Erickson, LA, Sebo, TJ, Rumilla, KM & Zhang, J 2015, 'The diagnostic utility of combination of HMGA2 and IMP3 qRT-PCR testing in thyroid neoplasms', Applied Immunohistochemistry and Molecular Morphology, vol. 23, no. 1, pp. 36-43.
Jin, Long ; Lloyd, Ricardo V. ; Henry, Michael R. ; Erickson, Lori A. ; Sebo, Thomas J. ; Rumilla, Kandelaria M. ; Zhang, Jun. / The diagnostic utility of combination of HMGA2 and IMP3 qRT-PCR testing in thyroid neoplasms. In: Applied Immunohistochemistry and Molecular Morphology. 2015 ; Vol. 23, No. 1. pp. 36-43.
@article{db5be5dad1bd44d88f74b48ccdb33177,
title = "The diagnostic utility of combination of HMGA2 and IMP3 qRT-PCR testing in thyroid neoplasms",
abstract = "The diagnosis of malignant thyroid tumors in some cytologic and histologic specimens remains challenging. Highmobility group A2 (HMGA2) expression and insulin-like growth factor II mRNA-binding protein-3 (IMP3) expression were evaluated by relative quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The aim of this study was to evaluate whether the combination of HMGA2 and IMP3 qRTPCR was diagnostically useful in differentiating benign from malignant thyroid neoplasms. Fine-needle aspiration (FNA) specimens from 120 patients including 56 benign lesions and 64 carcinomas were used. The available 80 corresponding formalinfixed paraffin-embedded (FFPE) thyroid tissues from 66 patients were also included in this study. HMGA2 and IMP3 expression levels were detected by qRT-PCR and reported as relative fold change after normalizing with a calibrator. The diagnostic utilities of HMGA2 and IMP3 qRT-PCR tests were evaluated individually and in combination. In FNA specimens, HMGA2 and IMP3 expression was consistently higher in thyroid malignancies compared with benign lesions in all subgroups except in H{\"u} rthle cell tumors. After exclusion of H{\"u} rthle cell tumors, the sensitivity was 90.2{\%} for HMGA2, 88.2{\%} for IMP3, and 98{\%} for HMGA2+IMP3; the specificity was 97.1{\%} for HMGA2, 79.4{\%} for IMP3, and 79.4{\%} for HMGA+IMP3. qRT-PCR data showed similar results in FFPE tissues: the sensitivity was 84.2{\%} for HMGA2, 85.7{\%} for IMP3, and 94.7{\%} for HMGA2+IMP3; the specificity was 96.9{\%} for HMGA2, 91.2{\%} for IMP3, and 90.6{\%} for HMGA2+IMP3. qRT-PCR data were concordant between FNA and FFPE samples for HMGA2 (97.4{\%}) and IMP3 (96.9{\%}). The results indicate that HMGA2 qRT-PCR with high specificity may be a useful ancillary technique to assist in the classification of difficult thyroid specimens, excluding H{\"u} rthle cell tumors. The HMGA2 and IMP3 qRTPCR combination model with increased sensitivity and negative predictive value (96.4{\%}) may be useful in screening thyroid cytology specimens.",
keywords = "HMGA2, IMP3, Quantitative RT-PCR, Thyroid",
author = "Long Jin and Lloyd, {Ricardo V.} and Henry, {Michael R.} and Erickson, {Lori A.} and Sebo, {Thomas J.} and Rumilla, {Kandelaria M.} and Jun Zhang",
year = "2015",
month = "1",
day = "14",
language = "English (US)",
volume = "23",
pages = "36--43",
journal = "Applied Immunohistochemistry and Molecular Morphology",
issn = "1541-2016",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - The diagnostic utility of combination of HMGA2 and IMP3 qRT-PCR testing in thyroid neoplasms

AU - Jin, Long

AU - Lloyd, Ricardo V.

AU - Henry, Michael R.

AU - Erickson, Lori A.

AU - Sebo, Thomas J.

AU - Rumilla, Kandelaria M.

AU - Zhang, Jun

PY - 2015/1/14

Y1 - 2015/1/14

N2 - The diagnosis of malignant thyroid tumors in some cytologic and histologic specimens remains challenging. Highmobility group A2 (HMGA2) expression and insulin-like growth factor II mRNA-binding protein-3 (IMP3) expression were evaluated by relative quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The aim of this study was to evaluate whether the combination of HMGA2 and IMP3 qRTPCR was diagnostically useful in differentiating benign from malignant thyroid neoplasms. Fine-needle aspiration (FNA) specimens from 120 patients including 56 benign lesions and 64 carcinomas were used. The available 80 corresponding formalinfixed paraffin-embedded (FFPE) thyroid tissues from 66 patients were also included in this study. HMGA2 and IMP3 expression levels were detected by qRT-PCR and reported as relative fold change after normalizing with a calibrator. The diagnostic utilities of HMGA2 and IMP3 qRT-PCR tests were evaluated individually and in combination. In FNA specimens, HMGA2 and IMP3 expression was consistently higher in thyroid malignancies compared with benign lesions in all subgroups except in Hü rthle cell tumors. After exclusion of Hü rthle cell tumors, the sensitivity was 90.2% for HMGA2, 88.2% for IMP3, and 98% for HMGA2+IMP3; the specificity was 97.1% for HMGA2, 79.4% for IMP3, and 79.4% for HMGA+IMP3. qRT-PCR data showed similar results in FFPE tissues: the sensitivity was 84.2% for HMGA2, 85.7% for IMP3, and 94.7% for HMGA2+IMP3; the specificity was 96.9% for HMGA2, 91.2% for IMP3, and 90.6% for HMGA2+IMP3. qRT-PCR data were concordant between FNA and FFPE samples for HMGA2 (97.4%) and IMP3 (96.9%). The results indicate that HMGA2 qRT-PCR with high specificity may be a useful ancillary technique to assist in the classification of difficult thyroid specimens, excluding Hü rthle cell tumors. The HMGA2 and IMP3 qRTPCR combination model with increased sensitivity and negative predictive value (96.4%) may be useful in screening thyroid cytology specimens.

AB - The diagnosis of malignant thyroid tumors in some cytologic and histologic specimens remains challenging. Highmobility group A2 (HMGA2) expression and insulin-like growth factor II mRNA-binding protein-3 (IMP3) expression were evaluated by relative quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The aim of this study was to evaluate whether the combination of HMGA2 and IMP3 qRTPCR was diagnostically useful in differentiating benign from malignant thyroid neoplasms. Fine-needle aspiration (FNA) specimens from 120 patients including 56 benign lesions and 64 carcinomas were used. The available 80 corresponding formalinfixed paraffin-embedded (FFPE) thyroid tissues from 66 patients were also included in this study. HMGA2 and IMP3 expression levels were detected by qRT-PCR and reported as relative fold change after normalizing with a calibrator. The diagnostic utilities of HMGA2 and IMP3 qRT-PCR tests were evaluated individually and in combination. In FNA specimens, HMGA2 and IMP3 expression was consistently higher in thyroid malignancies compared with benign lesions in all subgroups except in Hü rthle cell tumors. After exclusion of Hü rthle cell tumors, the sensitivity was 90.2% for HMGA2, 88.2% for IMP3, and 98% for HMGA2+IMP3; the specificity was 97.1% for HMGA2, 79.4% for IMP3, and 79.4% for HMGA+IMP3. qRT-PCR data showed similar results in FFPE tissues: the sensitivity was 84.2% for HMGA2, 85.7% for IMP3, and 94.7% for HMGA2+IMP3; the specificity was 96.9% for HMGA2, 91.2% for IMP3, and 90.6% for HMGA2+IMP3. qRT-PCR data were concordant between FNA and FFPE samples for HMGA2 (97.4%) and IMP3 (96.9%). The results indicate that HMGA2 qRT-PCR with high specificity may be a useful ancillary technique to assist in the classification of difficult thyroid specimens, excluding Hü rthle cell tumors. The HMGA2 and IMP3 qRTPCR combination model with increased sensitivity and negative predictive value (96.4%) may be useful in screening thyroid cytology specimens.

KW - HMGA2

KW - IMP3

KW - Quantitative RT-PCR

KW - Thyroid

UR - http://www.scopus.com/inward/record.url?scp=84917691331&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84917691331&partnerID=8YFLogxK

M3 - Article

VL - 23

SP - 36

EP - 43

JO - Applied Immunohistochemistry and Molecular Morphology

JF - Applied Immunohistochemistry and Molecular Morphology

SN - 1541-2016

IS - 1

ER -