The development of electrically conductive polycaprolactone fumarate-polypyrrole composite materials for nerve regeneration

M. Brett Runge, Mahrokh Dadsetan, Jonas Baltrusaitis, Andrew M. Knight, Terry Ruesink, Eric A. Lazcano, Lichun Lu, Anthony John Windebank, Michael J Yaszemski

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Electrically conductive polymer composites composed of polycaprolactone fumarate and polypyrrole (PCLF-PPy) have been developed for nerve regeneration applications. Here we report the synthesis and characterization of PCLF-PPy and in vitro studies showing PCLF-PPy materials support both PC12 cell and dorsal root ganglia (DRG) neurite extension. PCLF-PPy composite materials were synthesized by polymerizing pyrrole in preformed PCLF scaffolds (Mn 7,000 or 18,000 g mol-1) resulting in interpenetrating networks of PCLF-PPy. Chemical compositions and thermal properties were characterized by ATR-FTIR, XPS, DSC, and TGA. PCLF-PPy materials were synthesized with five different anions (naphthalene-2-sulfonic acid sodium salt (NSA), dodecylbenzenesulfonic acid sodium salt (DBSA), dioctyl sulfosuccinate sodium salt (DOSS), potassium iodide (I), and lysine) to investigate effects on electrical conductivity and to optimize chemical composition for cellular compatibility. PCLF-PPy materials have variable electrical conductivity up to 6 mS cm-1 with bulk compositions ranging from 5 to 13.5 percent polypyrrole. AFM and SEM characterization show microstructures with a root mean squared (RMS) roughness of 1195 nm and nanostructures with RMS roughness of 8 nm. In vitro studies using PC12 cells and DRG show PCLF-PPy materials synthesized with NSA or DBSA support cell attachment, proliferation, neurite extension, and are promising materials for future studies involving electrical stimulation.

Original languageEnglish (US)
Pages (from-to)5916-5926
Number of pages11
JournalBiomaterials
Volume31
Issue number23
DOIs
StatePublished - Aug 2010

Fingerprint

Polycaprolactone
Nerve Regeneration
Polypyrroles
Composite materials
Sodium
Salts
Electric Conductivity
PC12 Cells
Spinal Ganglia
Neurites
Acids
Surface roughness
Dioctyl Sulfosuccinic Acid
Chemical analysis
Potassium iodide
Potassium Iodide
Pyrroles
Interpenetrating polymer networks
poly(caprolactone fumarate)
polypyrrole

Keywords

  • Electrically conductive
  • Nerve
  • PCLF
  • Polypyrrole

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics
  • Medicine(all)

Cite this

The development of electrically conductive polycaprolactone fumarate-polypyrrole composite materials for nerve regeneration. / Brett Runge, M.; Dadsetan, Mahrokh; Baltrusaitis, Jonas; Knight, Andrew M.; Ruesink, Terry; Lazcano, Eric A.; Lu, Lichun; Windebank, Anthony John; Yaszemski, Michael J.

In: Biomaterials, Vol. 31, No. 23, 08.2010, p. 5916-5926.

Research output: Contribution to journalArticle

Brett Runge, M. ; Dadsetan, Mahrokh ; Baltrusaitis, Jonas ; Knight, Andrew M. ; Ruesink, Terry ; Lazcano, Eric A. ; Lu, Lichun ; Windebank, Anthony John ; Yaszemski, Michael J. / The development of electrically conductive polycaprolactone fumarate-polypyrrole composite materials for nerve regeneration. In: Biomaterials. 2010 ; Vol. 31, No. 23. pp. 5916-5926.
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