The cytoplasmic domain of the Drosophila cell adhesion molecule neuroglian is not essential for its homophilic adhesive properties in S2 cells

M. Hortsch, Y. M E Wang, Y. Marikar, A. J. Bieber

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Abstract

Drosophila neuroglian is a transmembrane glycoprotein that has strong structural and sequence homology to the vertebrate L1 gene family of cell adhesion molecules (Bieber, A. J., Snow, P.M., Hortsch, M., Patel, N. H., Jacobs, J. R., Traquina, Z. R., Schilling, J., and Goodman, C. S. (1989) Cell 59, 447-460). Two different neuroglian protein forms that are generated by a differential splicing process are expressed in a tissue-specific fashion by embryonic and larval cells (Hortsch, M., Bieber, A. J., Patel, N. H., and Goodman, C. S. (1990) Neuron 4, 697709). The two neuroglian polypeptides differ only in their cytoplasmic domains. Both of these neuroglian species, when transfected into and expressed in Drosophila S2 cells, induce the calcium-independent, homophilic aggregation of transformed cells. A third artificial neuroglian protein form was constructed by substituting the neuroglian transmembrane segment and cytoplasmic domains with the glycosyl phosphatidylinositol attachment signal of the Drosophila fasciclin I protein. This cDNA construct generates a glycosyl phosphatidylinositol-anchored form of neuroglian, which retains the ability to induce homophilic cell aggregation when expressed in S2 cells, and was able to interact with both of the two naturally occurring neuroglian polypeptides. These results demonstrate that neuroglian mediates a calcium-independent, homophilic cell adhesion activity and that neither cytoplasmic neuroglian domains nor a direct interaction with cytoskeletal elements is essential for this property.

Original languageEnglish (US)
Pages (from-to)18809-18817
Number of pages9
JournalJournal of Biological Chemistry
Volume270
Issue number32
DOIs
StatePublished - 1995
Externally publishedYes

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Glycosylphosphatidylinositols
Cell Adhesion Molecules
Adhesives
Drosophila
Agglomeration
Calcium
Cell Aggregation
Peptides
Cell adhesion
Neurons
Glycoproteins
Proteins
Complementary DNA
Genes
Tissue
Sequence Homology
Cell Adhesion
Vertebrates
fasciclin I

ASJC Scopus subject areas

  • Biochemistry

Cite this

The cytoplasmic domain of the Drosophila cell adhesion molecule neuroglian is not essential for its homophilic adhesive properties in S2 cells. / Hortsch, M.; Wang, Y. M E; Marikar, Y.; Bieber, A. J.

In: Journal of Biological Chemistry, Vol. 270, No. 32, 1995, p. 18809-18817.

Research output: Contribution to journalArticle

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