The crystal structure of the liver fatty acid-binding protein. A complex with two bound oleates

James Thompson, Nate Winter, Daniel Terwey, Judy Bratt, Leonard Banaszak

Research output: Contribution to journalArticle

210 Citations (Scopus)

Abstract

The crystal structure of the recombinant form of rat liver fatty acid- binding protein was completed to 2.3 Å and refined to an R factor of 19.0%. The structural solution was obtained by molecular replacement using superimposed polyalanine coordinates of six intracellular lipid-binding proteins as a search probe. The entire amino acid sequence of rat liver fatty acid-binding protein along with an amino-terminal formyl-methionine was modeled in the crystal structure. In addition, the crystal was obtained in the presence of oleic acid, and the initial electron density clearly showed two fatty acid molecules bound within a central cavity. The carboxylate of one fatty acid molecule interacts with arginine 122 and is shielded from free solvent. It has an overall bent conformation. The more solvent-exposed carboxylate of the other oleate is located near the helix-turn-helix that caps one end of the β-barrel, while the acyl chain lies in the interior. The cavity contains both polar and nonpolar residues but also shows extensive hydrophobic character around the nonpolar atoms of the ligands. The primary and secondary oleate binding sites appear to be totally interdependent, mainly because favorable hydrophobic interactions form between both aliphatic chains.

Original languageEnglish (US)
Pages (from-to)7140-7150
Number of pages11
JournalJournal of Biological Chemistry
Volume272
Issue number11
DOIs
StatePublished - Mar 14 1997
Externally publishedYes

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Fatty Acid-Binding Proteins
Oleic Acid
Crystal structure
Rats
Fatty Acids
Molecules
R Factors
Hydrophobic and Hydrophilic Interactions
Methionine
Carrier concentration
Arginine
Conformations
Amino Acid Sequence
Carrier Proteins
Binding Sites
Electrons
Ligands
Lipids
Amino Acids
Atoms

ASJC Scopus subject areas

  • Biochemistry

Cite this

The crystal structure of the liver fatty acid-binding protein. A complex with two bound oleates. / Thompson, James; Winter, Nate; Terwey, Daniel; Bratt, Judy; Banaszak, Leonard.

In: Journal of Biological Chemistry, Vol. 272, No. 11, 14.03.1997, p. 7140-7150.

Research output: Contribution to journalArticle

Thompson, James ; Winter, Nate ; Terwey, Daniel ; Bratt, Judy ; Banaszak, Leonard. / The crystal structure of the liver fatty acid-binding protein. A complex with two bound oleates. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 11. pp. 7140-7150.
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