TY - JOUR
T1 - The contribution of HLA class I antigens in immune status following two doses of rubella vaccination
AU - Ovsyannikova, Inna G.
AU - Jacobson, Robert M.
AU - Vierkant, Robert A.
AU - Jacobsen, Steven J.
AU - Pankratz, V. Shane
AU - Poland, Gregory A.
N1 - Funding Information:
We thank the parents and children who participated in this study. We acknowledge the efforts of the fellows, nurses, and students from the Mayo Vaccine Research Group. We thank Tina Agostini for her excellent skills in performing HLA typings. We thank Neelam Dhiman, Norman A. Pinsky, and Rawleigh C. Howe for developing and performing assays, and we thank Shaun D. Maloney for assistance with statistical analysis. We gratefully acknowledge Kim S. Zabel for her editorial assistance in preparing this manuscript. This work was supported by grants AI 33144 and AI 48793 from the National Institutes of Health.
PY - 2004/12
Y1 - 2004/12
N2 - The variability of humoral and cellular immune responses modulated by human leukocyte antigen (HLA) genes is a significant factor in the protective effect of rubella vaccines. We performed HLA class I typing in a group of 346 healthy schoolchildren and young adults who previously received two doses of measles-mumps-rubella-II vaccine. Rubella virus-specific humoral (serum antibody) immunity and cell-mediated immunity (lymphocyte proliferation) were assessed. Median values for antibody levels and stimulation indices (SI) were 38.63 IU/ml and 2.29 IU/ml, respectively. The alleles that provided suggestive, but not conclusive, evidence of HLA association with rubella seropositivity were HLA-B*2705 (median, 24.68 IU/ml; p = 0.160), B*4501 (median, 61.22 IU/ml; p = 0.098), Cw*0303 (median, 30.34 IU/ml; p = 0.102) and Cw*0704 (median, 26.58 IU/ml; p = 0.144). These alleles approach, but do not achieve, statistical significance. Of all the alleles analyzed, HLA-B*3503 (median SI, 3.00; p = 0.031) and HLA-Cw*1502 (median SI, 3.19; p = 0.035) were positively associated with lymphoproliferative responses to rubella virus antigens, whereas the HLA-B*3901 (SI, 1.34; p = 0.066) allele was negatively associated. This suggests that class I HLA alleles may have limited associations with humoral and cellular immune responses to rubella vaccine. These data may facilitate our understanding of immune response variation in a genetically outbred heterogeneous population.
AB - The variability of humoral and cellular immune responses modulated by human leukocyte antigen (HLA) genes is a significant factor in the protective effect of rubella vaccines. We performed HLA class I typing in a group of 346 healthy schoolchildren and young adults who previously received two doses of measles-mumps-rubella-II vaccine. Rubella virus-specific humoral (serum antibody) immunity and cell-mediated immunity (lymphocyte proliferation) were assessed. Median values for antibody levels and stimulation indices (SI) were 38.63 IU/ml and 2.29 IU/ml, respectively. The alleles that provided suggestive, but not conclusive, evidence of HLA association with rubella seropositivity were HLA-B*2705 (median, 24.68 IU/ml; p = 0.160), B*4501 (median, 61.22 IU/ml; p = 0.098), Cw*0303 (median, 30.34 IU/ml; p = 0.102) and Cw*0704 (median, 26.58 IU/ml; p = 0.144). These alleles approach, but do not achieve, statistical significance. Of all the alleles analyzed, HLA-B*3503 (median SI, 3.00; p = 0.031) and HLA-Cw*1502 (median SI, 3.19; p = 0.035) were positively associated with lymphoproliferative responses to rubella virus antigens, whereas the HLA-B*3901 (SI, 1.34; p = 0.066) allele was negatively associated. This suggests that class I HLA alleles may have limited associations with humoral and cellular immune responses to rubella vaccine. These data may facilitate our understanding of immune response variation in a genetically outbred heterogeneous population.
KW - HLA class I
KW - antibody and lymphoproliferative responses
KW - rubella vaccine
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U2 - 10.1016/j.humimm.2004.07.001
DO - 10.1016/j.humimm.2004.07.001
M3 - Article
C2 - 15603879
AN - SCOPUS:10444252525
VL - 65
SP - 1506
EP - 1515
JO - Human Immunology
JF - Human Immunology
SN - 0198-8859
IS - 12
ER -