The complete primary structure of the human snRNP E protein

David R. Stanford, Maria Kehl, Caroline A. Perry, Eileen L. Holicky, Scott E. Harvey, Anne M. Rohleder, Kai Render, Reinhard Luhrmann, Eric D. Wieben

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The snRNP E protein is one of four "core" proteins associated with the snRNAs of the U family (U1,U2,U4,U5, and U6). Screening of a human teratoma cDNA library with a partial cDNA for a human autoimmune antigen resulted in the isolation of a cDNA clone containing the entire coding region of this snRNP core protein. Comparison of the 5′ end of this cDNA with the sequences of two processed pseudogenes and primer extension data suggest that the cDNA is nearly full length. The longest open reading frame in this clone codes for a basic 92 amino acid protein which is in perfect agreement with amino acid sequence data obtained from purified E protein. The predicted sequence of this protein reveals no extensive similarity to other snRNP proteins, but contains regions of similarity to a eukaryotic ribosomal protein.

Original languageEnglish (US)
Pages (from-to)10593-10605
Number of pages13
JournalNucleic acids research
Volume16
Issue number22
DOIs
StatePublished - Nov 25 1988

ASJC Scopus subject areas

  • Genetics

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