The centrin-based cytoskeleton of Chlamydomonas reinhardtii

Distribution in interphase and mitotic cells

Jeffrey L Salisbury, A. T. Baron, M. A. Sanders

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

Monoclonal and polyclonal antibodies raised against algal centrin, a protein of algal striated flagellar roots, were used to characterize the occurrence and distribution of this protein in interphase and mitotic Chlamydomonas cells. Chlamydomonas centrin, as identified by Western immunoblot procedures, is a low molecular (20,000-M(r)) acidic protein. Immunofluorescence and immunogold labeling demonstrates that centrin is a component of the distal fiber. In addition, centrin-based flagellar roots link the flagellar apparatus to the nucleus. Two major descending fibers extend from the basal bodies toward the nucleus; each descending fiber branches several times giving rise to 8-16 fimbria which surround and embrace the nucleus. Immunogold labeling indicates that these fimbria are juxtaposed to the outer nuclear envelope. Earlier studies have demonstrated that the centrin-based linkage between the flagellar apparatus and the nucleus is contractile, both in vitro and in living Chlamydomonas cells (Wright, R.L., J. Salisbury, and J. Jarvik. 1985. J. Cell Biol. 101:1903-1912; Salisbury, J. L., M.A. Sanders, and L. Harpst. 1987. J. Cell Biol. 105:1799-1805). Immunofluorescence studies show dramatic changes in distribution of the centrin-based system during mitosis that include a transient contraction at preprophase; division, separation, and re-extenion during prophase; and a second transient contraction at the metaphase/anaphase boundary. These observations suggest a fundamental role for centrin in motile events during mitosis.

Original languageEnglish (US)
Pages (from-to)635-641
Number of pages7
JournalJournal of Cell Biology
Volume107
Issue number2
StatePublished - 1988
Externally publishedYes

Fingerprint

Chlamydomonas reinhardtii
Interphase
Sulfamethoxazole Drug Combination Trimethoprim
Cytoskeleton
Chlamydomonas
Mitosis
Fluorescent Antibody Technique
Algal Proteins
Basal Bodies
Prophase
Anaphase
Nuclear Envelope
Metaphase
Proteins
Western Blotting
Monoclonal Antibodies

ASJC Scopus subject areas

  • Cell Biology

Cite this

The centrin-based cytoskeleton of Chlamydomonas reinhardtii : Distribution in interphase and mitotic cells. / Salisbury, Jeffrey L; Baron, A. T.; Sanders, M. A.

In: Journal of Cell Biology, Vol. 107, No. 2, 1988, p. 635-641.

Research output: Contribution to journalArticle

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