The CD3ε proline-rich sequence, and its interaction with Nck, is not required for T cell development and function

Andrea L. Szymczak, Creg J. Workman, Diana Gil, Smaroula Dilioglou, Kate M. Vignali, Ed Palmer, Dario A.A. Vignali

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

The CD3ε proline-rich sequence (PRS) binds to the cytosolic adaptor molecule Nck after TCR ligation. It has been proposed that this interaction is essential for immunological synapse formation and T cell activation. To assess the physiological importance of the CD3ε PRS, we have generated mice that lack this motif (CD3ε.PRSM). Pull-down experiments demonstrated the inability of Nck to bind to the CD3ε PRS in thymocytes from mutant mice after TCR ligation. Surprisingly, no differences were observed in the number and percentage of T cell subsets in the thymus and spleen, and there was no apparent defect in positive or negative selection. Furthermore, the proliferative response of CD3ε.PRSM T cells to staphylococcal enterotoxin B and anti-CD3 Ab was normal. TCR surface expression, constitutive internalization, and Ag-induced down-modulation were also normal. These data suggest that the interaction between the CD3ε PRS and Nck, or any other Src homology 3 domain-containing molecule, is not essential for T cell development and function.

Original languageEnglish (US)
Pages (from-to)270-275
Number of pages6
JournalJournal of Immunology
Volume175
Issue number1
DOIs
StatePublished - Jul 1 2005

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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