The Ca2+-transporting ATPases of rabbit and trout exhibit different pH- and temperature dependences

Eduardo Nunes Chini, F. G S De Toledo, M. C. Albuquerque, L. De Meis

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The phosphorylation of the trout sarcoplasmic-reticulum Ca2+-ATPase by P(i) differs in its temperature- and pH-dependence from the rabbit ATPase. In the trout enzyme, the apparent affinity for P(i) and maximum phosphoenzyme values do not vary over a pH and temperature ranges that have a pronounced effect on the rabbit enzyme. The lack of temperature-dependence for phosphorylation is observed at pH 6.8. At pH 8.0, the temperature profile for phosphorylation of the trout enzyme resembles that of the rabbit at pH 6.8. The rabbit ATPase is no longer phosphorylated by P(i) after solubilization with the detergent C12E9. In contrast, the trout enzyme can be phosphorylated by P(i) after solubilization with C(l2)E9, and the same levels of phosphoenzyme were obtained with the soluble and membrane-bound ATPase at both 0° and 25°C. In the range of 0-20 °C, the rates of ATP synthesis and of Ca2+ uptake by the trout ATPase are less temperature-dependent than for the rabbit enzyme. However, both isoenzymes catalyse ATP hydrolysis with similar temperature-dependences. The results raise the possibility that protonation of specific amino acid residues may contribute to the lack of temperature-dependence for phosphorylation of the trout Ca2+-ATPase.

Original languageEnglish (US)
Pages (from-to)469-473
Number of pages5
JournalBiochemical Journal
Volume293
Issue number2
StatePublished - 1993
Externally publishedYes

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Calcium-Transporting ATPases
Trout
Adenosine Triphosphatases
Phosphorylation
Rabbits
Temperature
Enzymes
Adenosine Triphosphate
Protonation
Sarcoplasmic Reticulum
Detergents
Isoenzymes
Hydrolysis
Membranes
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

The Ca2+-transporting ATPases of rabbit and trout exhibit different pH- and temperature dependences. / Chini, Eduardo Nunes; De Toledo, F. G S; Albuquerque, M. C.; De Meis, L.

In: Biochemical Journal, Vol. 293, No. 2, 1993, p. 469-473.

Research output: Contribution to journalArticle

Chini, EN, De Toledo, FGS, Albuquerque, MC & De Meis, L 1993, 'The Ca2+-transporting ATPases of rabbit and trout exhibit different pH- and temperature dependences', Biochemical Journal, vol. 293, no. 2, pp. 469-473.
Chini, Eduardo Nunes ; De Toledo, F. G S ; Albuquerque, M. C. ; De Meis, L. / The Ca2+-transporting ATPases of rabbit and trout exhibit different pH- and temperature dependences. In: Biochemical Journal. 1993 ; Vol. 293, No. 2. pp. 469-473.
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