The BRCT Domain Is a Phospho-Protein Binding Domain

Xiaochun Yu, Claudia Christiano Silva Chini, Miao He, Georges Mer, Junjie Chen

Research output: Contribution to journalArticle

627 Scopus citations

Abstract

The carboxyl-terminal domain (BRCT) of the Breast Cancer Gene 1 (BRCA1) protein is an evolutionarily conserved module that exists in a large number of proteins from prokaryotes to eukaryotes. Although most BRCT domain-containing proteins participate in DNA-damage checkpoint or DNA-repair pathways, or both, the function of the BRCT domain is not fully understood. We show that the BRCA1 BRCT domain directly interacts with phosphorylated BRCA1-Associated Carboxyl-terminal Helicase (BACH1). This specific interaction between BRCA1 and phosphorylated BACH1 is cell cycle regulated and is required for DNA damage-induced checkpoint control during the transition from G2 to M phase of the cell cycle. Further, we show that two other BRCT domains interact with their respective physiological partners in a phosphorylation-dependent manner. Thirteen additional BRCT domains also preferentially bind phospho-peptides rather than nonphosphorylated control peptides. These data imply that the BRCT domain is a phospho-protein binding domain involved in cell cycle control.

Original languageEnglish (US)
Pages (from-to)639-642
Number of pages4
JournalScience
Volume302
Issue number5645
DOIs
StatePublished - Oct 24 2003

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    Yu, X., Chini, C. C. S., He, M., Mer, G., & Chen, J. (2003). The BRCT Domain Is a Phospho-Protein Binding Domain. Science, 302(5645), 639-642. https://doi.org/10.1126/science.1088753