The BRCT Domain Is a Phospho-Protein Binding Domain

Xiaochun Yu, Claudia Christiano Silva Chini, Miao He, Georges Mer, Junjie Chen

Research output: Contribution to journalArticle

605 Citations (Scopus)

Abstract

The carboxyl-terminal domain (BRCT) of the Breast Cancer Gene 1 (BRCA1) protein is an evolutionarily conserved module that exists in a large number of proteins from prokaryotes to eukaryotes. Although most BRCT domain-containing proteins participate in DNA-damage checkpoint or DNA-repair pathways, or both, the function of the BRCT domain is not fully understood. We show that the BRCA1 BRCT domain directly interacts with phosphorylated BRCA1-Associated Carboxyl-terminal Helicase (BACH1). This specific interaction between BRCA1 and phosphorylated BACH1 is cell cycle regulated and is required for DNA damage-induced checkpoint control during the transition from G2 to M phase of the cell cycle. Further, we show that two other BRCT domains interact with their respective physiological partners in a phosphorylation-dependent manner. Thirteen additional BRCT domains also preferentially bind phospho-peptides rather than nonphosphorylated control peptides. These data imply that the BRCT domain is a phospho-protein binding domain involved in cell cycle control.

Original languageEnglish (US)
Pages (from-to)639-642
Number of pages4
JournalScience
Volume302
Issue number5645
DOIs
StatePublished - Oct 24 2003

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Neoplasm Genes
Protein Binding
Breast Neoplasms
DNA Damage
Cell Cycle
BRCA1 Protein
Peptides
Cell Cycle Checkpoints
Eukaryota
DNA Repair
Cell Division
Proteins
Phosphorylation
Protein Domains

ASJC Scopus subject areas

  • General

Cite this

Yu, X., Chini, C. C. S., He, M., Mer, G., & Chen, J. (2003). The BRCT Domain Is a Phospho-Protein Binding Domain. Science, 302(5645), 639-642. https://doi.org/10.1126/science.1088753

The BRCT Domain Is a Phospho-Protein Binding Domain. / Yu, Xiaochun; Chini, Claudia Christiano Silva; He, Miao; Mer, Georges; Chen, Junjie.

In: Science, Vol. 302, No. 5645, 24.10.2003, p. 639-642.

Research output: Contribution to journalArticle

Yu, X, Chini, CCS, He, M, Mer, G & Chen, J 2003, 'The BRCT Domain Is a Phospho-Protein Binding Domain', Science, vol. 302, no. 5645, pp. 639-642. https://doi.org/10.1126/science.1088753
Yu, Xiaochun ; Chini, Claudia Christiano Silva ; He, Miao ; Mer, Georges ; Chen, Junjie. / The BRCT Domain Is a Phospho-Protein Binding Domain. In: Science. 2003 ; Vol. 302, No. 5645. pp. 639-642.
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