The blood B-cells and bone marrow plasma cells in patients with multiple myeloma share identical IgH rearrangements

P. L. Bergsagel, A. Masellis Smith, A. R. Belch, L. M. Pilarski

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Previous reports have described the phenotypic and functional properties of monotypic late stage B cells in the blood of patients with multiple myeloma and have speculated that these B cells represent a malignant circulating component of myeloma. Here we show that blood B cells have IgH rearrangements identical to those expressed by the bone marrow plasma cells by using Ig Fingerprint and Allele-Specific Oligomer (ASO) polymerase chain reaction (PCR) methods. DNA from purified blood B cells and bone marrow plasma cells taken at the same time, and blood B cells taken at subsequent patient visits was amplified using consensus IgH primers, or ASO primers. In 10/16 patients, a single IgH rearrangement was amplified from the bone marrow plasma cells. In all 10 of those patients the same clonotypic rearrangement was amplified from the purified blood B cells. The relationship of these clonal blood B cells to the malignant bone marrow plasma cells remains undetermined.

Original languageEnglish (US)
Pages (from-to)17-24
Number of pages8
JournalCurrent Topics in Microbiology and Immunology
Volume194
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)

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