The bioelectrical basis and validity of gastrointestinal extracellular slow wave recordings

Timothy R. Angeli, Peng Du, Niranchan Paskaranandavadivel, Patrick W M Janssen, Arthur Beyder, Roger G. Lentle, Ian P. Bissett, Leo K. Cheng, Gregory O'Grady

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Gastrointestinal extracellular recordings have been a core technique in motility research for a century. However, the bioelectrical basis of extracellular data has recently been challenged by claims that these techniques preferentially assay movement artifacts, cannot reproduce the underlying slow wave kinetics, and misrepresent the true slow wave frequency. These claims motivated this joint experimental-theoretical study, which aimed to define the sources and validity of extracellular potentials. In vivo extracellular recordings and video capture were performed in the porcine jejunum, before and after intra-arterial nifedipine administration. Gastric extracellular recordings were recorded simultaneously using conventional serosal contact and suction electrodes, and biphasic and monophasic extracellular potentials were simulated in a biophysical model. Contractions were abolished by nifedipine, but extracellular slow waves persisted, with unchanged amplitude, downstroke rate, velocity, and downstroke width (P > 0.10 for all), at reduced frequency (24% lower; P= 0.03). Simultaneous suction and conventional serosal extracellular recordings were identical in phase (frequency and activation-recovery interval), but varied in morphology (monophasic vs. biphasic; downstroke rate and amplitude: P < 0.0001). Simulations demonstrated the field contribution of current flow to extracellular potential and quantified the effects of localised depolarisation due to suction pressure on extracellular potential morphology. In sum, these results demonstrate that gastrointestinal extracellular slow wave recordings cannot be explained by motion artifacts, and are of a bioelectrical origin that is highly consistent with the underlying biophysics of slow wave propagation. Motion suppression is shown to be unnecessary as a routine control in in vivo extracellular studies, supporting the validity of the extant gastrointestinal extracellular literature.

Original languageEnglish (US)
Pages (from-to)4567-4579
Number of pages13
JournalJournal of Physiology
Volume591
Issue number18
DOIs
StatePublished - Sep 2013

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Suction
Nifedipine
Artifacts
Biophysics
Video Recording
Jejunum
Stomach
Electrodes
Theoretical Models
Swine
Pressure
Research

ASJC Scopus subject areas

  • Physiology

Cite this

Angeli, T. R., Du, P., Paskaranandavadivel, N., Janssen, P. W. M., Beyder, A., Lentle, R. G., ... O'Grady, G. (2013). The bioelectrical basis and validity of gastrointestinal extracellular slow wave recordings. Journal of Physiology, 591(18), 4567-4579. https://doi.org/10.1113/jphysiol.2013.254292

The bioelectrical basis and validity of gastrointestinal extracellular slow wave recordings. / Angeli, Timothy R.; Du, Peng; Paskaranandavadivel, Niranchan; Janssen, Patrick W M; Beyder, Arthur; Lentle, Roger G.; Bissett, Ian P.; Cheng, Leo K.; O'Grady, Gregory.

In: Journal of Physiology, Vol. 591, No. 18, 09.2013, p. 4567-4579.

Research output: Contribution to journalArticle

Angeli, TR, Du, P, Paskaranandavadivel, N, Janssen, PWM, Beyder, A, Lentle, RG, Bissett, IP, Cheng, LK & O'Grady, G 2013, 'The bioelectrical basis and validity of gastrointestinal extracellular slow wave recordings', Journal of Physiology, vol. 591, no. 18, pp. 4567-4579. https://doi.org/10.1113/jphysiol.2013.254292
Angeli, Timothy R. ; Du, Peng ; Paskaranandavadivel, Niranchan ; Janssen, Patrick W M ; Beyder, Arthur ; Lentle, Roger G. ; Bissett, Ian P. ; Cheng, Leo K. ; O'Grady, Gregory. / The bioelectrical basis and validity of gastrointestinal extracellular slow wave recordings. In: Journal of Physiology. 2013 ; Vol. 591, No. 18. pp. 4567-4579.
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