The Aurora A-HP1γ pathway regulates gene expression and mitosis in cells from the sperm lineage

Phoebe H. Leonard, Adrienne Grzenda, Angela Mathison, Dean E. Morbeck, Jolene R. Fredrickson, Thiago M. De Assuncao, Trace Christensen, Jeffrey L Salisbury, Ezequiel Calvo, Juan Iovanna, Charles C. Coddington, Raul Urrutia, Gwen Lomberk

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: HP1γ, a well-known regulator of gene expression, has been recently identified to be a target of Aurora A, a mitotic kinase which is important for both gametogenesis and embryogenesis. The purpose of this study was to define whether the Aurora A-HP1γ pathway supports cell division of gametes and/or early embryos, using western blot, immunofluorescence, immunohistochemistry, electron microscopy, shRNA-based knockdown, site-directed mutagenesis, and Affymetrix-based genome-wide expression profiles. Results: We find that the form of HP1γ phosphorylated by Aurora A, P-Ser83 HP1γ, is a passenger protein, which localizes to the spermatozoa centriole and axoneme. In addition, disruption in this pathway causes centrosomal abnormalities and aberrations in cell division. Expression profiling of male germ cell lines demonstrates that HP1γ phosphorylation is critical for the regulation of mitosis-associated gene expression networks. In female gametes, we observe that P-Ser83-HP1γ is not present in meiotic centrosomes of M2 oocytes, but after syngamy, it becomes detectable during cleavage divisions, coinciding with early embryonic genome activation. Conclusions: These results support the idea that phosphorylation of HP1γ by Aurora A plays a role in the regulation of gene expression and mitotic cell division in cells from the sperm lineage and in early embryos. Combined, this data is relevant to better understanding the function of HP1γ in reproductive biology.

Original languageEnglish (US)
Article number23
JournalBMC Developmental Biology
Volume15
Issue number1
DOIs
StatePublished - May 29 2015

Fingerprint

Cell Lineage
Mitosis
Germ Cells
Cell Division
Spermatozoa
Gene Expression
Embryonic Structures
Phosphorylation
Genome
Axoneme
Gametogenesis
Centrioles
Centrosome
Gene Regulatory Networks
Gene Expression Regulation
Regulator Genes
Site-Directed Mutagenesis
Small Interfering RNA
Oocytes
Embryonic Development

Keywords

  • CBX3
  • Embryonic genome activation
  • Epigenetics
  • Gametes
  • Heterochromatin Protein 1
  • HP1γ
  • Meiosis
  • Mitosis
  • Preimplantation embryo

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Leonard, P. H., Grzenda, A., Mathison, A., Morbeck, D. E., Fredrickson, J. R., De Assuncao, T. M., ... Lomberk, G. (2015). The Aurora A-HP1γ pathway regulates gene expression and mitosis in cells from the sperm lineage. BMC Developmental Biology, 15(1), [23]. https://doi.org/10.1186/s12861-015-0073-x

The Aurora A-HP1γ pathway regulates gene expression and mitosis in cells from the sperm lineage. / Leonard, Phoebe H.; Grzenda, Adrienne; Mathison, Angela; Morbeck, Dean E.; Fredrickson, Jolene R.; De Assuncao, Thiago M.; Christensen, Trace; Salisbury, Jeffrey L; Calvo, Ezequiel; Iovanna, Juan; Coddington, Charles C.; Urrutia, Raul; Lomberk, Gwen.

In: BMC Developmental Biology, Vol. 15, No. 1, 23, 29.05.2015.

Research output: Contribution to journalArticle

Leonard, PH, Grzenda, A, Mathison, A, Morbeck, DE, Fredrickson, JR, De Assuncao, TM, Christensen, T, Salisbury, JL, Calvo, E, Iovanna, J, Coddington, CC, Urrutia, R & Lomberk, G 2015, 'The Aurora A-HP1γ pathway regulates gene expression and mitosis in cells from the sperm lineage', BMC Developmental Biology, vol. 15, no. 1, 23. https://doi.org/10.1186/s12861-015-0073-x
Leonard PH, Grzenda A, Mathison A, Morbeck DE, Fredrickson JR, De Assuncao TM et al. The Aurora A-HP1γ pathway regulates gene expression and mitosis in cells from the sperm lineage. BMC Developmental Biology. 2015 May 29;15(1). 23. https://doi.org/10.1186/s12861-015-0073-x
Leonard, Phoebe H. ; Grzenda, Adrienne ; Mathison, Angela ; Morbeck, Dean E. ; Fredrickson, Jolene R. ; De Assuncao, Thiago M. ; Christensen, Trace ; Salisbury, Jeffrey L ; Calvo, Ezequiel ; Iovanna, Juan ; Coddington, Charles C. ; Urrutia, Raul ; Lomberk, Gwen. / The Aurora A-HP1γ pathway regulates gene expression and mitosis in cells from the sperm lineage. In: BMC Developmental Biology. 2015 ; Vol. 15, No. 1.
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AU - Christensen, Trace

AU - Salisbury, Jeffrey L

AU - Calvo, Ezequiel

AU - Iovanna, Juan

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AU - Lomberk, Gwen

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