The Association of Peripheral Blood Regulatory T-Cell Concentrations with Epithelial Ovarian Cancer: A Brief Report

Rikki A. Cannioto, Lara E. Sucheston-Campbell, Shalaka Hampras, Ellen L Goode, Keith L Knutson, Roberta Ness, Francesmary Modugno, Paul K. Wallace, J. Brian Szender, Paul Mayor, Chi Chen Hong, Janine M. Joseph, Grace Friel, Warren Davis, Mary Nesline, Kevin H. Eng, Robert P. Edwards, Bridget Kruszka, Kristina Schmitt, Kunle OdunsiKirsten B. Moysich

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective There is a mounting body of evidence demonstrating higher percentages of regulatory T (Treg) cells in the peripheral blood of patients with cancer in comparison to healthy controls, but there is a paucity of epidemiological literature characterizing circulating Treg cells among patients with epithelial ovarian cancer (EOC). To investigate the role of peripheral Treg cells in ovarian neoplasms, we conducted a case-control study to characterize circulating concentrations of Treg cells among patients with EOC, women with benign ovarian conditions, and healthy controls without a history of cancer. Materials and Methods Participants were identified for inclusion due to their participation in the Data Bank and BioRepository program at Roswell Park Cancer Institute in Buffalo, NY. Patients included 71 women with a primary diagnosis of EOC and 195 women with a diagnosis of benign ovarian conditions. Controls included 101 age- and race-matched women without a history of cancer. Nonfasting, pretreatment peripheral blood levels of CD3+CD4+CD25+FOXP3+ Treg cells were measured using flow cytometric analyses and expressed as a percentage of total CD3+ cells and as a percentage of total CD3+CD4+ cells. Results Compared to healthy controls and women with benign ovarian conditions, patients with EOC had significantly higher frequency of Treg cells (P < 0.04). In multivariable logistic regression analyses using Treg frequency expressed as a percentage of CD+3 cells, we observed a significant positive association between Treg cell percentage and EOC risk, with each 1% increase associated with a 37% increased risk of EOC (odds ratio, 1.37; 95% confidence interval, 1.04-1.80). We observed a similar trend when Treg frequency was expressed as a percentage of CD3+CD+4 cells (odds ratio, 1.22; 95% confidence interval, 0.99-1.49). Conclusions The current study provides support that peripheral Treg cell frequency is elevated in patients with EOC in comparison to women with benign ovarian conditions and healthy controls.

Original languageEnglish (US)
Pages (from-to)11-16
Number of pages6
JournalInternational Journal of Gynecological Cancer
Volume27
Issue number1
DOIs
StatePublished - Jan 1 2017

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Regulatory T-Lymphocytes
Neoplasms
Odds Ratio
Confidence Intervals
Ovarian epithelial cancer
Buffaloes
Ovarian Neoplasms
Case-Control Studies
Logistic Models
Regression Analysis
Databases

Keywords

  • Epithelial ovarian cancer
  • T-regulatory cells
  • Treg cells

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

Cite this

The Association of Peripheral Blood Regulatory T-Cell Concentrations with Epithelial Ovarian Cancer : A Brief Report. / Cannioto, Rikki A.; Sucheston-Campbell, Lara E.; Hampras, Shalaka; Goode, Ellen L; Knutson, Keith L; Ness, Roberta; Modugno, Francesmary; Wallace, Paul K.; Szender, J. Brian; Mayor, Paul; Hong, Chi Chen; Joseph, Janine M.; Friel, Grace; Davis, Warren; Nesline, Mary; Eng, Kevin H.; Edwards, Robert P.; Kruszka, Bridget; Schmitt, Kristina; Odunsi, Kunle; Moysich, Kirsten B.

In: International Journal of Gynecological Cancer, Vol. 27, No. 1, 01.01.2017, p. 11-16.

Research output: Contribution to journalArticle

Cannioto, RA, Sucheston-Campbell, LE, Hampras, S, Goode, EL, Knutson, KL, Ness, R, Modugno, F, Wallace, PK, Szender, JB, Mayor, P, Hong, CC, Joseph, JM, Friel, G, Davis, W, Nesline, M, Eng, KH, Edwards, RP, Kruszka, B, Schmitt, K, Odunsi, K & Moysich, KB 2017, 'The Association of Peripheral Blood Regulatory T-Cell Concentrations with Epithelial Ovarian Cancer: A Brief Report', International Journal of Gynecological Cancer, vol. 27, no. 1, pp. 11-16. https://doi.org/10.1097/IGC.0000000000000845
Cannioto, Rikki A. ; Sucheston-Campbell, Lara E. ; Hampras, Shalaka ; Goode, Ellen L ; Knutson, Keith L ; Ness, Roberta ; Modugno, Francesmary ; Wallace, Paul K. ; Szender, J. Brian ; Mayor, Paul ; Hong, Chi Chen ; Joseph, Janine M. ; Friel, Grace ; Davis, Warren ; Nesline, Mary ; Eng, Kevin H. ; Edwards, Robert P. ; Kruszka, Bridget ; Schmitt, Kristina ; Odunsi, Kunle ; Moysich, Kirsten B. / The Association of Peripheral Blood Regulatory T-Cell Concentrations with Epithelial Ovarian Cancer : A Brief Report. In: International Journal of Gynecological Cancer. 2017 ; Vol. 27, No. 1. pp. 11-16.
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T1 - The Association of Peripheral Blood Regulatory T-Cell Concentrations with Epithelial Ovarian Cancer

T2 - A Brief Report

AU - Cannioto, Rikki A.

AU - Sucheston-Campbell, Lara E.

AU - Hampras, Shalaka

AU - Goode, Ellen L

AU - Knutson, Keith L

AU - Ness, Roberta

AU - Modugno, Francesmary

AU - Wallace, Paul K.

AU - Szender, J. Brian

AU - Mayor, Paul

AU - Hong, Chi Chen

AU - Joseph, Janine M.

AU - Friel, Grace

AU - Davis, Warren

AU - Nesline, Mary

AU - Eng, Kevin H.

AU - Edwards, Robert P.

AU - Kruszka, Bridget

AU - Schmitt, Kristina

AU - Odunsi, Kunle

AU - Moysich, Kirsten B.

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N2 - Objective There is a mounting body of evidence demonstrating higher percentages of regulatory T (Treg) cells in the peripheral blood of patients with cancer in comparison to healthy controls, but there is a paucity of epidemiological literature characterizing circulating Treg cells among patients with epithelial ovarian cancer (EOC). To investigate the role of peripheral Treg cells in ovarian neoplasms, we conducted a case-control study to characterize circulating concentrations of Treg cells among patients with EOC, women with benign ovarian conditions, and healthy controls without a history of cancer. Materials and Methods Participants were identified for inclusion due to their participation in the Data Bank and BioRepository program at Roswell Park Cancer Institute in Buffalo, NY. Patients included 71 women with a primary diagnosis of EOC and 195 women with a diagnosis of benign ovarian conditions. Controls included 101 age- and race-matched women without a history of cancer. Nonfasting, pretreatment peripheral blood levels of CD3+CD4+CD25+FOXP3+ Treg cells were measured using flow cytometric analyses and expressed as a percentage of total CD3+ cells and as a percentage of total CD3+CD4+ cells. Results Compared to healthy controls and women with benign ovarian conditions, patients with EOC had significantly higher frequency of Treg cells (P < 0.04). In multivariable logistic regression analyses using Treg frequency expressed as a percentage of CD+3 cells, we observed a significant positive association between Treg cell percentage and EOC risk, with each 1% increase associated with a 37% increased risk of EOC (odds ratio, 1.37; 95% confidence interval, 1.04-1.80). We observed a similar trend when Treg frequency was expressed as a percentage of CD3+CD+4 cells (odds ratio, 1.22; 95% confidence interval, 0.99-1.49). Conclusions The current study provides support that peripheral Treg cell frequency is elevated in patients with EOC in comparison to women with benign ovarian conditions and healthy controls.

AB - Objective There is a mounting body of evidence demonstrating higher percentages of regulatory T (Treg) cells in the peripheral blood of patients with cancer in comparison to healthy controls, but there is a paucity of epidemiological literature characterizing circulating Treg cells among patients with epithelial ovarian cancer (EOC). To investigate the role of peripheral Treg cells in ovarian neoplasms, we conducted a case-control study to characterize circulating concentrations of Treg cells among patients with EOC, women with benign ovarian conditions, and healthy controls without a history of cancer. Materials and Methods Participants were identified for inclusion due to their participation in the Data Bank and BioRepository program at Roswell Park Cancer Institute in Buffalo, NY. Patients included 71 women with a primary diagnosis of EOC and 195 women with a diagnosis of benign ovarian conditions. Controls included 101 age- and race-matched women without a history of cancer. Nonfasting, pretreatment peripheral blood levels of CD3+CD4+CD25+FOXP3+ Treg cells were measured using flow cytometric analyses and expressed as a percentage of total CD3+ cells and as a percentage of total CD3+CD4+ cells. Results Compared to healthy controls and women with benign ovarian conditions, patients with EOC had significantly higher frequency of Treg cells (P < 0.04). In multivariable logistic regression analyses using Treg frequency expressed as a percentage of CD+3 cells, we observed a significant positive association between Treg cell percentage and EOC risk, with each 1% increase associated with a 37% increased risk of EOC (odds ratio, 1.37; 95% confidence interval, 1.04-1.80). We observed a similar trend when Treg frequency was expressed as a percentage of CD3+CD+4 cells (odds ratio, 1.22; 95% confidence interval, 0.99-1.49). Conclusions The current study provides support that peripheral Treg cell frequency is elevated in patients with EOC in comparison to women with benign ovarian conditions and healthy controls.

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KW - T-regulatory cells

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