The association between circulating MicroRNA levels and coronary endothelial function

R. Jay Widmer, Woo Young Chung, Joerg Herrmann, Kyra L. Jordan, Lilach O Lerman, Amir Lerman

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Human microRNAs (miRs) have been implicated in human diseases presumably through the downregulation and silencing of targeted genes via post-translational modifications. However, their role in the early stage of coronary atherosclerosis is not known. The aim of this study was to test the hypothesis that patients with early atherosclerosis and coronary endothelial dysfunction (CED) have alterations in transcoronary miR gradients. Patients underwent coronary angiography and endothelial function testing in the cardiac catheterization laboratory. Patients were divided into abnormal (n = 26) and normal (n = 22) microvascular coronary endothelial function based on intracoronary response to infused acetylcholine measured as a percent change in coronary blood flow (CBF) and arterial diameter. Blood samples were obtained simultaneously from the aorta and coronary sinus at the time of catheterization for RNA isolation, and miR subsequently assessed. Baseline characteristics were similar in both groups. Patients with microvascular CED displayed transcoronary gradients significantly elevated in miR-92a and miR-133 normalized to C-elegans-39 miR. Percent change in CBF and the transcoronary gradient of miR-133 displayed a significant inverse correlation (r2 = 0.11, p = 0.03). Thus, we present novel data whereupon selected miRs demonstrate elevated transcoronary gradients in patients with microvascular CED. The current findings support further studies on the mechanistic role of miRs in coronary atherosclerosis and in humans.

Original languageEnglish (US)
Article numbere109650
JournalPLoS One
Volume9
Issue number10
DOIs
StatePublished - Oct 13 2014

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MicroRNAs
microRNA
Blood
Coronary Artery Disease
Angiography
catheters
blood flow
coronary sinus
Acetylcholine
Genes
RNA
Coronary Sinus
post-translational modification
Gene Silencing
acetylcholine
Post Translational Protein Processing
Cardiac Catheterization
aorta
atherosclerosis
Coronary Angiography

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The association between circulating MicroRNA levels and coronary endothelial function. / Widmer, R. Jay; Chung, Woo Young; Herrmann, Joerg; Jordan, Kyra L.; Lerman, Lilach O; Lerman, Amir.

In: PLoS One, Vol. 9, No. 10, e109650, 13.10.2014.

Research output: Contribution to journalArticle

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