TY - JOUR
T1 - The antigenic structure of the human glycoprotein hormone α-subunit
T2 - II. Cross-species comparisons
AU - Bergert, Elizabeth R.
AU - Madden, Benjamin
AU - McCormick, Daniel J.
AU - Papkoff, Harold
AU - Ryan, Robert J.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1990/12
Y1 - 1990/12
N2 - Eight monoclonal antibodies, specific for the glycoprotein hormone α-subunit, were raised against human free α-subunit, human FSH, or human CG. All of these antihuman monoclonal antibodies were tested for cross-reactivity with α-subunits derived from bovine, porcine, equine, bull frog, sea turtle, turkey, and ostrich glycoprotein hormones. All showed cross-reactivity with affinities ranging from 10-4 to 10-8 depending upon the antibody and the species of α-subunit. Cyanogen bromide fragments of bovine and equine α, when tested with selected antibodies indicated that antigenic determinants could be localized in two regions: α9-33 and α76-92. Comparison of amino acid sequences, and relative potencies, suggest that major antigenic determinants involve residues 21, 22, and 23 (F-F-S in human α) and 76-85 (G-G-F-K-V-E-N-H-T-A in human α). As part of this study the N-terminal amino acid sequences of bull frog, sea turtle, turkey, and ostrich α-subunits were determined and reported for the first time.
AB - Eight monoclonal antibodies, specific for the glycoprotein hormone α-subunit, were raised against human free α-subunit, human FSH, or human CG. All of these antihuman monoclonal antibodies were tested for cross-reactivity with α-subunits derived from bovine, porcine, equine, bull frog, sea turtle, turkey, and ostrich glycoprotein hormones. All showed cross-reactivity with affinities ranging from 10-4 to 10-8 depending upon the antibody and the species of α-subunit. Cyanogen bromide fragments of bovine and equine α, when tested with selected antibodies indicated that antigenic determinants could be localized in two regions: α9-33 and α76-92. Comparison of amino acid sequences, and relative potencies, suggest that major antigenic determinants involve residues 21, 22, and 23 (F-F-S in human α) and 76-85 (G-G-F-K-V-E-N-H-T-A in human α). As part of this study the N-terminal amino acid sequences of bull frog, sea turtle, turkey, and ostrich α-subunits were determined and reported for the first time.
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M3 - Article
C2 - 1701134
AN - SCOPUS:0025674119
SN - 0013-7227
VL - 127
SP - 2985
EP - 2989
JO - Endocrinology
JF - Endocrinology
IS - 6
ER -