The androgen receptor of the testicular-feminized (Tfm) mutant mouse is smaller than the wild-type receptor

C. Y F Young, M. P. Johnson, J. L. Prescott, D. J. Tindall

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The physicochemical and immunological properties of androgen receptors from kidney and brain of testicular-feminized (Tfm) mutant mice and wild-type mice were compared. Analysis by gel filtration and sucrose density gradients revealed that the mol wt of the mutant receptor was 66K (38Å; 3.8S) which was significantly smaller than the 110K (53Å; 4.6S) size of the wild-type androgen receptor (P < 0.05). Mixing experiments failed to demonstrate any role for differential proteolysis in the size differences between these receptors. Interaction of the mutant androgen receptor with specific polyclonal antiandrogen receptor antibodies produced significantly smaller immune complexes than that formed with wild-type receptor (12S vs. 17S; P < 0.01). This confirmed the smaller size of the Tfm mutant androgen receptor and suggested that it contained fewer epitopes. The Tfm kidney cytosols also demonstrated a decreased concentration of androgen receptor-binding activity relative to that of the wild type. Together, these results suggest that the androgen insensitivity associated with the Tfm phenotype is due to a deficiency of androgen receptor in target tissues and qualitative defect in the androgen receptor protein itself.

Original languageEnglish (US)
Pages (from-to)771-775
Number of pages5
JournalEndocrinology
Volume124
Issue number2
StatePublished - 1989
Externally publishedYes

Fingerprint

Androgen Receptors
Androgen-Insensitivity Syndrome
Kidney
Androgen Antagonists
Antigen-Antibody Complex
Cytosol
Androgens
Proteolysis
Gel Chromatography
Sucrose
Epitopes
Phenotype
Antibodies
Brain
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Young, C. Y. F., Johnson, M. P., Prescott, J. L., & Tindall, D. J. (1989). The androgen receptor of the testicular-feminized (Tfm) mutant mouse is smaller than the wild-type receptor. Endocrinology, 124(2), 771-775.

The androgen receptor of the testicular-feminized (Tfm) mutant mouse is smaller than the wild-type receptor. / Young, C. Y F; Johnson, M. P.; Prescott, J. L.; Tindall, D. J.

In: Endocrinology, Vol. 124, No. 2, 1989, p. 771-775.

Research output: Contribution to journalArticle

Young, CYF, Johnson, MP, Prescott, JL & Tindall, DJ 1989, 'The androgen receptor of the testicular-feminized (Tfm) mutant mouse is smaller than the wild-type receptor', Endocrinology, vol. 124, no. 2, pp. 771-775.
Young, C. Y F ; Johnson, M. P. ; Prescott, J. L. ; Tindall, D. J. / The androgen receptor of the testicular-feminized (Tfm) mutant mouse is smaller than the wild-type receptor. In: Endocrinology. 1989 ; Vol. 124, No. 2. pp. 771-775.
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