The acute phase protein haptoglobin regulates host immunity

Kristin M. Huntoon, Yanping Wang, Cheryl A. Eppolito, Karen W. Barbour, Franklin G. Berger, Protul A. Shrikant, Heinz Baumann

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The contribution of acute phase plasma proteins to host immune responses remains poorly characterized. To better understand the role of the acute phase reactant and major hemoglobin-binding protein haptoglobin (Hp) on the function of immune cells, we generated Hp-deficient C57BL/6J mice. These mice exhibit stunted development of lymphoid organs associated with lower counts of mature T and B cells in the blood and secondary lymphoid compartments. Moreover, these mice show markedly reduced adaptive immune responses as represented by reduced accumulation of IgG antibody after immunization with adjuvant and nominal antigen, abrogation of Th1-dominated delayed-type hypersensitivity reaction, loss of mitogenic responses mounted by T cells, and reduced T cell responses conveyed by APCs. Collectively, these defects are in agreement with the observations that Hp-deficient mice are not capable of generating a recall response or deterring a Salmonella infection as well as failing to generate tumor antigen-specific responses. The administration of Hp to lymphocytes in tissue culture partially ameliorates these functional defects, lending further support to our contention that the acute phase response protein Hp has the ability to regulate immune cell responses and host immunity. The phenotype of Hp-deficient mice suggests a major regulatory activity for Hp in supporting proliferation and functional differentiation of B and T cells as part of homeostasis and in response to antigen stimulation.

Original languageEnglish (US)
Pages (from-to)170-181
Number of pages12
JournalJournal of Leukocyte Biology
Volume84
Issue number1
DOIs
StatePublished - Jul 1 2008
Externally publishedYes

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Haptoglobins
Acute-Phase Proteins
Immunity
T-Lymphocytes
B-Lymphocytes
Antigens
Acute-Phase Reaction
Salmonella Infections
Delayed Hypersensitivity
Adaptive Immunity
Neoplasm Antigens
Inbred C57BL Mouse
Blood Proteins
Immunization
Carrier Proteins
Hemoglobins
Homeostasis
Immunoglobulin G
Lymphocytes
Phenotype

Keywords

  • Dendritic cell
  • Humoral immune response
  • T cell

ASJC Scopus subject areas

  • Cell Biology

Cite this

Huntoon, K. M., Wang, Y., Eppolito, C. A., Barbour, K. W., Berger, F. G., Shrikant, P. A., & Baumann, H. (2008). The acute phase protein haptoglobin regulates host immunity. Journal of Leukocyte Biology, 84(1), 170-181. https://doi.org/10.1189/jlb.0208100

The acute phase protein haptoglobin regulates host immunity. / Huntoon, Kristin M.; Wang, Yanping; Eppolito, Cheryl A.; Barbour, Karen W.; Berger, Franklin G.; Shrikant, Protul A.; Baumann, Heinz.

In: Journal of Leukocyte Biology, Vol. 84, No. 1, 01.07.2008, p. 170-181.

Research output: Contribution to journalArticle

Huntoon, KM, Wang, Y, Eppolito, CA, Barbour, KW, Berger, FG, Shrikant, PA & Baumann, H 2008, 'The acute phase protein haptoglobin regulates host immunity', Journal of Leukocyte Biology, vol. 84, no. 1, pp. 170-181. https://doi.org/10.1189/jlb.0208100
Huntoon KM, Wang Y, Eppolito CA, Barbour KW, Berger FG, Shrikant PA et al. The acute phase protein haptoglobin regulates host immunity. Journal of Leukocyte Biology. 2008 Jul 1;84(1):170-181. https://doi.org/10.1189/jlb.0208100
Huntoon, Kristin M. ; Wang, Yanping ; Eppolito, Cheryl A. ; Barbour, Karen W. ; Berger, Franklin G. ; Shrikant, Protul A. ; Baumann, Heinz. / The acute phase protein haptoglobin regulates host immunity. In: Journal of Leukocyte Biology. 2008 ; Vol. 84, No. 1. pp. 170-181.
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