TY - JOUR
T1 - The accuracy of Qβ RNA translation
T2 - 1. Errors during the synthesis of Qβ proteins by intact Escherichia coli cells
AU - KHAZAIE, Khashayarsha
AU - BUCHANAN, John H.
AU - ROSENBERGER, Robert F.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1984/11
Y1 - 1984/11
N2 - The fidelity of Qβ RNA translation by intact Escherichia coli cells has been studied. After infection, host protein synthesis was eliminated by adding rifampicin and the radioactive, phage‐specified, proteins separated by one or two‐dimensional gel electrophoresis. Labelled histidine and tryptophan were incorporated into the phage coat protein, whose message does not specify these amino acids, at a frequency of 0.09–0.13 per molecule. Errors leading to a change in the pi of the coat protein occurred at a rate of 0.05 per molecule, while the coat protein UGA stop codon was misread 6.5% of the time. These error rates are similar to data in some recent publications but much higher than the canonical 3–4 × 10−4. They further provide a reference point in vivo to which the translation of the same message by E. coli extracts can be compared.
AB - The fidelity of Qβ RNA translation by intact Escherichia coli cells has been studied. After infection, host protein synthesis was eliminated by adding rifampicin and the radioactive, phage‐specified, proteins separated by one or two‐dimensional gel electrophoresis. Labelled histidine and tryptophan were incorporated into the phage coat protein, whose message does not specify these amino acids, at a frequency of 0.09–0.13 per molecule. Errors leading to a change in the pi of the coat protein occurred at a rate of 0.05 per molecule, while the coat protein UGA stop codon was misread 6.5% of the time. These error rates are similar to data in some recent publications but much higher than the canonical 3–4 × 10−4. They further provide a reference point in vivo to which the translation of the same message by E. coli extracts can be compared.
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U2 - 10.1111/j.1432-1033.1984.tb08491.x
DO - 10.1111/j.1432-1033.1984.tb08491.x
M3 - Article
C2 - 6386472
AN - SCOPUS:0021753875
SN - 0014-2956
VL - 144
SP - 485
EP - 489
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 3
ER -