In this study, we use antisera to synthetic β-amyloid protein precursor (βAPP) peptides to identify, in human brain and cerebrospinal fluid (CSF), soluble ≃125- and ≃105-kDa derivatives of the βAPP that lack the carboxyl terminus of the full-length, membrane-associated forms. We show that the soluble ≃125-kDa βAPP derivative contains the Kunitz protease inhibitor domain, whereas the ≃105-kDa form does not, and we confirm that these two proteins are soluble βAPP derivatives by purifying each from human CSF and directly sequencing its amino terminus.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - Jan 1 1989|
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