The β-amyloid protein precursor of Alzheimer disease has soluble derivatives found in human brain and cerebrospinal fluid

M. R. Palmert, M. B. Podlisny, D. S. Witker, T. Oltersdorf, L. H. Younkin, D. J. Selkoe, S. G. Younkin

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Abstract

In this study, we use antisera to synthetic β-amyloid protein precursor (βAPP) peptides to identify, in human brain and cerebrospinal fluid (CSF), soluble ≃125- and ≃105-kDa derivatives of the βAPP that lack the carboxyl terminus of the full-length, membrane-associated forms. We show that the soluble ≃125-kDa βAPP derivative contains the Kunitz protease inhibitor domain, whereas the ≃105-kDa form does not, and we confirm that these two proteins are soluble βAPP derivatives by purifying each from human CSF and directly sequencing its amino terminus.

Original languageEnglish (US)
Pages (from-to)6338-6342
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number16
DOIs
StatePublished - Jan 1 1989

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