The β-amyloid protein precursor of Alzheimer disease has soluble derivatives found in human brain and cerebrospinal fluid

M. R. Palmert; M. B. Podlisny; D. S. Witker; T. Oltersdorf; L. H. Younkin; D. J. Selkoe; S. G. Younkin

doi:10.1073/pnas.86.16.6338

The β-amyloid protein precursor of Alzheimer disease has soluble derivatives found in human brain and cerebrospinal fluid

M. R. Palmert, M. B. Podlisny, D. S. Witker, T. Oltersdorf, L. H. Younkin, D. J. Selkoe, S. G. Younkin

Neuroscience

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214 Scopus citations

Abstract

In this study, we use antisera to synthetic β-amyloid protein precursor (βAPP) peptides to identify, in human brain and cerebrospinal fluid (CSF), soluble ≃125- and ≃105-kDa derivatives of the βAPP that lack the carboxyl terminus of the full-length, membrane-associated forms. We show that the soluble ≃125-kDa βAPP derivative contains the Kunitz protease inhibitor domain, whereas the ≃105-kDa form does not, and we confirm that these two proteins are soluble βAPP derivatives by purifying each from human CSF and directly sequencing its amino terminus.

Original language	English (US)
Pages (from-to)	6338-6342
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	86
Issue number	16
DOIs	https://doi.org/10.1073/pnas.86.16.6338
State	Published - 1989

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Palmert, M. R., Podlisny, M. B., Witker, D. S., Oltersdorf, T., Younkin, L. H., Selkoe, D. J., & Younkin, S. G. (1989). The β-amyloid protein precursor of Alzheimer disease has soluble derivatives found in human brain and cerebrospinal fluid. Proceedings of the National Academy of Sciences of the United States of America, 86(16), 6338-6342. https://doi.org/10.1073/pnas.86.16.6338

Palmert, MR, Podlisny, MB, Witker, DS, Oltersdorf, T, Younkin, LH, Selkoe, DJ & Younkin, SG 1989, 'The β-amyloid protein precursor of Alzheimer disease has soluble derivatives found in human brain and cerebrospinal fluid', Proceedings of the National Academy of Sciences of the United States of America, vol. 86, no. 16, pp. 6338-6342. https://doi.org/10.1073/pnas.86.16.6338

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The β-amyloid protein precursor of Alzheimer disease has soluble derivatives found in human brain and cerebrospinal fluid

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