TY - JOUR
T1 - TG-I
T2 - A marker for neuronal nuclei in Alzheimer's disease
AU - Vincent, Inez
AU - Mattiace, Linda A.
AU - Dickson, Dennis W.
AU - Rosado, Michelle
AU - Katen, Roger
AU - Davies, Peter
N1 - Funding Information:
valuable suggestions on the manuscript.This work was supported by grants, NIMH 38623 and AG06803.
PY - 1994
Y1 - 1994
N2 - TG-1 is a monoclonal antibody (mAb) raised against paired helical filaments purified from Alzheimer's Disease (AD) brain by immunoaffinity chromatography. By immunocytochemistry, TG-1 reveals abundant staining of neuronal nuclei in AD brain, but little or no staining in normal brain. TG-1 stained nuclei are observed in areas of AD brain with neurofibrillary pathology and in certain neurones that are not normally affected. Biochemical studies with TG-1 show antigens of 32-38 kDa in pellets and 50 kDa in supernatants from brain, with no obvious differences between normal and AD. TG-1 also recognizes an unusual structure, i.e., a 'starburst' in brain tissue from AD and elderly normals. Starbursts are not immunoreactive for the astrocytic marker, glial fibrillary acidic protein, and are not associated with amyloid. Widespread nuclear staining is observed with TG-1 in rat brain, and the immunoreactive antigens in purified nuclei are similar to those in human brain. Thus, TG-1 identifies neuronal nuclear antigens that are altered in AD, and provides a new avenue for studying pathogenic mechanisms in the disease.
AB - TG-1 is a monoclonal antibody (mAb) raised against paired helical filaments purified from Alzheimer's Disease (AD) brain by immunoaffinity chromatography. By immunocytochemistry, TG-1 reveals abundant staining of neuronal nuclei in AD brain, but little or no staining in normal brain. TG-1 stained nuclei are observed in areas of AD brain with neurofibrillary pathology and in certain neurones that are not normally affected. Biochemical studies with TG-1 show antigens of 32-38 kDa in pellets and 50 kDa in supernatants from brain, with no obvious differences between normal and AD. TG-1 also recognizes an unusual structure, i.e., a 'starburst' in brain tissue from AD and elderly normals. Starbursts are not immunoreactive for the astrocytic marker, glial fibrillary acidic protein, and are not associated with amyloid. Widespread nuclear staining is observed with TG-1 in rat brain, and the immunoreactive antigens in purified nuclei are similar to those in human brain. Thus, TG-1 identifies neuronal nuclear antigens that are altered in AD, and provides a new avenue for studying pathogenic mechanisms in the disease.
KW - Dentate gyrus granule cells
KW - Microtubule-associated tau protein
KW - Neurofibrillary pathology
KW - Nuclear protein kinases
KW - Paired helical filaments
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U2 - 10.1006/nbdi.1994.0018
DO - 10.1006/nbdi.1994.0018
M3 - Article
C2 - 9173994
AN - SCOPUS:0028677474
SN - 0969-9961
VL - 1
SP - 145
EP - 157
JO - Neurobiology of Disease
JF - Neurobiology of Disease
IS - 3
ER -