TY - JOUR
T1 - TFAR19 gene changes the biophysical properties of murine erythroleukemia cells
AU - Gu, Li
AU - Jiang, Yuhui
AU - Wang, Ying
AU - Yao, Weijuan
AU - Sun, Dagong
AU - Ka, Weibo
AU - Tang, Zhiyu
AU - Xu, Xiaofeng
AU - Wen, Zongyao
AU - Chien, Shu
N1 - Funding Information:
This work was supported by two National Natural Science Foundations of China (grants numbers 30270355 and 10172009) and a Doctoral Foundation grant number 20010001082.
PY - 2005/11
Y1 - 2005/11
N2 - TFAR19 is a novel apoptosis-related gene and can accelerate cell apoptosis in the presence of apoptosis inducements. Here, we studied the effects of TFAR19 on some biophysical properties of mouse erythroleukemia (MEL) cells and their molecular and structural basis. After transfected with TFAR19 and apoptosis inducement, MEL revealed a high cell membrane fluidity, a decrease in resynthesis of phospholipids, an increase in the proteins/nucleic acids ratio, a relatively orderly cytoskeleton network, an impaired deformability a low integrin aM expression, and a decrease in adhesion to endothelial cells. These findings suggest the potential of TFAR19 for antitumor cell migration, and thus for antitumor gene therapy.
AB - TFAR19 is a novel apoptosis-related gene and can accelerate cell apoptosis in the presence of apoptosis inducements. Here, we studied the effects of TFAR19 on some biophysical properties of mouse erythroleukemia (MEL) cells and their molecular and structural basis. After transfected with TFAR19 and apoptosis inducement, MEL revealed a high cell membrane fluidity, a decrease in resynthesis of phospholipids, an increase in the proteins/nucleic acids ratio, a relatively orderly cytoskeleton network, an impaired deformability a low integrin aM expression, and a decrease in adhesion to endothelial cells. These findings suggest the potential of TFAR19 for antitumor cell migration, and thus for antitumor gene therapy.
KW - Adhesion
KW - Apoptosis
KW - Deformability
KW - Murine erythroleukemia cell line (MEL)
KW - Programmed cell death 5 (PDCD5)
KW - TF-1 cells apoptosis-related gene 19 (TFAR19)
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U2 - 10.1385/CBB:43:3:355
DO - 10.1385/CBB:43:3:355
M3 - Article
C2 - 16247177
AN - SCOPUS:27144520011
SN - 1085-9195
VL - 43
SP - 355
EP - 363
JO - Cell Biochemistry and Biophysics
JF - Cell Biochemistry and Biophysics
IS - 3
ER -