Tex14, a Plk1-Regulated Protein, Is Required for Kinetochore-Microtubule Attachment and Regulation of the Spindle Assembly Checkpoint

Gourish Mondal, Akihiro Ohashi, Lin Yang, Matthew Rowley, Fergus J Couch

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Proper assembly of kinetochores (KTs) during mitosis is required for bipolar attachment of spindle microtubules (MTs) and the accumulation of spindle assembly checkpoint (SAC) components. Here we show that testis-expressed protein 14 (Tex14), which has been implicated in midbody function, is recruited to KTs by Plk1 in a Cdk1-dependent manner during early mitosis. Exclusion of Tex14 from kinetochores results in an inability to efficiently localize outer KT components, impaired KT-MT attachment, chromosome congression defects, and whole-chromosome instability. In addition, we demonstrate that phosphorylation of Tex14 by Plk1 during metaphase promotes APCCdc20-mediated Tex14 degradation. Inhibition of this phosphorylation event causes retention of Tex14 at KTs and results in delayed metaphase-to-anaphase transition and chromosome segregation defects. Our findings identify Tex14 as an important mediator of KT structure and function and the fidelity of chromosome separation.

Original languageEnglish (US)
Pages (from-to)680-695
Number of pages16
JournalMolecular Cell
Volume45
Issue number5
DOIs
StatePublished - Mar 9 2012

Fingerprint

M Phase Cell Cycle Checkpoints
Kinetochores
Microtubules
Testis
Proteins
Metaphase
Mitosis
Chromosomes
Phosphorylation
Chromosomal Instability
Chromosome Segregation
Anaphase
polo-like kinase 1
Proteolysis

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Tex14, a Plk1-Regulated Protein, Is Required for Kinetochore-Microtubule Attachment and Regulation of the Spindle Assembly Checkpoint. / Mondal, Gourish; Ohashi, Akihiro; Yang, Lin; Rowley, Matthew; Couch, Fergus J.

In: Molecular Cell, Vol. 45, No. 5, 09.03.2012, p. 680-695.

Research output: Contribution to journalArticle

Mondal, Gourish ; Ohashi, Akihiro ; Yang, Lin ; Rowley, Matthew ; Couch, Fergus J. / Tex14, a Plk1-Regulated Protein, Is Required for Kinetochore-Microtubule Attachment and Regulation of the Spindle Assembly Checkpoint. In: Molecular Cell. 2012 ; Vol. 45, No. 5. pp. 680-695.
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