TEThered to Runx: Novel binding partners for runx factors

Xiaodong Li, Matthew Decker, Jennifer J. Westendorf

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

RUNX transcription factors reside in the nuclear matrix where they integrate numerous signaling pathways to regulate gene expression and affect tissue development, regeneration, and tumorigenesis. An affinity purification and proteomic experiment was performed to identify novel Runx2 binding partners. The interactions between Runx2 and two nuclear factors (Ddx5 and CoAA) identified in this screen were previously described. Coactivator activator (CoAA) bound the DNA binding domain of Runx2 and prevented Runx-driven gene expression. The YxxQ motif in CoAA was required for Runx2 interactions. Members of the FET/TET family of proteins, including FUS/TLS and EWSR1, contain a similar motif and were hypothesized to interact with Runx2. Here, we provide evidence that FUS/TLS, EWSR1, and the Ewing's sarcoma t(12;21) fusion protein EWS-FLI bind Runx2 and alter its transcriptional activity. Potential roles of protein complexes containing FET/TET and RUNX family members during tumor formation and mesenchymal progenitor cell differentiation are discussed.

Original languageEnglish (US)
Pages (from-to)82-85
Number of pages4
JournalBlood Cells, Molecules, and Diseases
Volume45
Issue number1
DOIs
StatePublished - Jun 2010

Keywords

  • CoAA
  • EWS
  • EWS-FLI
  • Ewing's sarcoma
  • FUS
  • TLS

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Hematology
  • Cell Biology

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