Testis-specific murine centrin, Cetn1: Genomic characterization and evidence for retroposition of a gene encoding a centrosome protein

Peter E. Hart, Janel N. Glantz, James D. Orth, Gregory M. Poynter, Jeffrey L Salisbury

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Centrin is a centrosome component in species from yeast to humans. Here, the mouse centrin 1 gene (Cetn1) is analyzed with respect to its genomic structure, chromosome localization, tissue-specific expression, and phylogenetic relationship to the other mouse centrin genes and their human orthologs. Cetn1 is an intronless gene located on chromosome 18A2 that encodes a 172-amino-acid protein with a predicted molecular mass of 19,696 Da (pI 4.61) and all of the structural features common to centrin. Cetn1 possesses the sequence features of an expressed retroposon: the gene lacks introns, the open reading frame is not interrupted by stop codons, and the coding region is flanked by a pair of direct repeats. Reverse transcriptase- polymerase chain reaction and Northern blot analysis demonstrate that Cetn1 expression is limited exclusively to the testis in adult male mice. Cetn1 expression is first seen in the neonatal testis at 14 days postpartum, reaching adult levels by day 17. These observations provide new insight into the regulation, function, and evolutionary history of centrin in higher eukaryotes.

Original languageEnglish (US)
Pages (from-to)111-120
Number of pages10
JournalGenomics
Volume60
Issue number2
DOIs
StatePublished - Sep 1 1999

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Centrosome
Sulfamethoxazole Drug Combination Trimethoprim
Testis
Genes
Proteins
Chromosome Structures
Gene Expression
Retroelements
Terminator Codon
Nucleic Acid Repetitive Sequences
Eukaryota
Reverse Transcriptase Polymerase Chain Reaction
Northern Blotting
Introns
Postpartum Period
Open Reading Frames
Chromosomes
Yeasts
Amino Acids

ASJC Scopus subject areas

  • Genetics

Cite this

Testis-specific murine centrin, Cetn1 : Genomic characterization and evidence for retroposition of a gene encoding a centrosome protein. / Hart, Peter E.; Glantz, Janel N.; Orth, James D.; Poynter, Gregory M.; Salisbury, Jeffrey L.

In: Genomics, Vol. 60, No. 2, 01.09.1999, p. 111-120.

Research output: Contribution to journalArticle

Hart, Peter E. ; Glantz, Janel N. ; Orth, James D. ; Poynter, Gregory M. ; Salisbury, Jeffrey L. / Testis-specific murine centrin, Cetn1 : Genomic characterization and evidence for retroposition of a gene encoding a centrosome protein. In: Genomics. 1999 ; Vol. 60, No. 2. pp. 111-120.
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