Tenogenic differentiation of human bone marrow stem cells via a combinatory effect of aligned chitosan-poly-caprolactone nanofibers and TGF-β3

Matthew Leung, Soumen Jana, Ching Ting Tsao, Miqin Zhang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Tendon injury occurs frequently and tendon repair is limited by its poor self-healing. The current tissue engineering approach for treating tendon injuries has showed limited success, largely due to the lack of scaffolds with suitable structural and biological properties, and suitable growth factors for differentiation of stem cells into tendon cells. This study investigated if the combination of environmental and biological cues from aligned chitosan-poly-caprolactone (C-PCL) combined with TGF-β3 growth factor can efficiently and rapidly direct the tenogenic differentiation of primary human bone marrow stem cells (BMSCs). C-PCL nanofibers were prepared to have the anisotropic nanostructure, and mechanical and biological properties similar to those of the native tendon extracellular matrix (ECM). The tenogenic commitment of BMSCs was assessed using cell morphology, and gene and protein expressions. BMSCs grown on uniaxially aligned C-PCL nanofibers in a medium containing TGF-β3 displayed an elongated morphology along nanofiber orientation, upregulated expressions of marker genes, and increased collagen production associated with tenogenic differentiation as compared to control substrates. Significantly, this tenogenic microenvironment induced the transcription of tenogenic markers in 5 days and production of a large amount of Collagen I in 10 days, more effective and faster than existing scaffolds combined with growth factors. This research reveals that a combinative effect of aligned C-PCL nanofibers and TGF-β3, as environmental and biological cues, can lead to rapid, effective BMSC differentiation into tenogenic progenitors, offering a potential strategy for managing tendon disorders.

Original languageEnglish (US)
Pages (from-to)6516-6524
Number of pages9
JournalJournal of Materials Chemistry B
Volume1
Issue number47
DOIs
StatePublished - Dec 21 2013
Externally publishedYes

Fingerprint

Nanofibers
Tendons
Chitosan
Stem cells
Bone Marrow Cells
Bone
Stem Cells
Tendon Injuries
Cues
Intercellular Signaling Peptides and Proteins
Collagen
Scaffolds
Growth Differentiation Factors
Gene Expression
Genes
Nanostructures
Tissue Engineering
Extracellular Matrix
Transcription
Cell Differentiation

ASJC Scopus subject areas

  • Chemistry(all)
  • Medicine(all)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

Tenogenic differentiation of human bone marrow stem cells via a combinatory effect of aligned chitosan-poly-caprolactone nanofibers and TGF-β3. / Leung, Matthew; Jana, Soumen; Tsao, Ching Ting; Zhang, Miqin.

In: Journal of Materials Chemistry B, Vol. 1, No. 47, 21.12.2013, p. 6516-6524.

Research output: Contribution to journalArticle

@article{bfe082b5f43e4645b830de6a8fe93b72,
title = "Tenogenic differentiation of human bone marrow stem cells via a combinatory effect of aligned chitosan-poly-caprolactone nanofibers and TGF-β3",
abstract = "Tendon injury occurs frequently and tendon repair is limited by its poor self-healing. The current tissue engineering approach for treating tendon injuries has showed limited success, largely due to the lack of scaffolds with suitable structural and biological properties, and suitable growth factors for differentiation of stem cells into tendon cells. This study investigated if the combination of environmental and biological cues from aligned chitosan-poly-caprolactone (C-PCL) combined with TGF-β3 growth factor can efficiently and rapidly direct the tenogenic differentiation of primary human bone marrow stem cells (BMSCs). C-PCL nanofibers were prepared to have the anisotropic nanostructure, and mechanical and biological properties similar to those of the native tendon extracellular matrix (ECM). The tenogenic commitment of BMSCs was assessed using cell morphology, and gene and protein expressions. BMSCs grown on uniaxially aligned C-PCL nanofibers in a medium containing TGF-β3 displayed an elongated morphology along nanofiber orientation, upregulated expressions of marker genes, and increased collagen production associated with tenogenic differentiation as compared to control substrates. Significantly, this tenogenic microenvironment induced the transcription of tenogenic markers in 5 days and production of a large amount of Collagen I in 10 days, more effective and faster than existing scaffolds combined with growth factors. This research reveals that a combinative effect of aligned C-PCL nanofibers and TGF-β3, as environmental and biological cues, can lead to rapid, effective BMSC differentiation into tenogenic progenitors, offering a potential strategy for managing tendon disorders.",
author = "Matthew Leung and Soumen Jana and Tsao, {Ching Ting} and Miqin Zhang",
year = "2013",
month = "12",
day = "21",
doi = "10.1039/c3tb20825g",
language = "English (US)",
volume = "1",
pages = "6516--6524",
journal = "Journal of Materials Chemistry B",
issn = "2050-7518",
publisher = "Royal Society of Chemistry",
number = "47",

}

TY - JOUR

T1 - Tenogenic differentiation of human bone marrow stem cells via a combinatory effect of aligned chitosan-poly-caprolactone nanofibers and TGF-β3

AU - Leung, Matthew

AU - Jana, Soumen

AU - Tsao, Ching Ting

AU - Zhang, Miqin

PY - 2013/12/21

Y1 - 2013/12/21

N2 - Tendon injury occurs frequently and tendon repair is limited by its poor self-healing. The current tissue engineering approach for treating tendon injuries has showed limited success, largely due to the lack of scaffolds with suitable structural and biological properties, and suitable growth factors for differentiation of stem cells into tendon cells. This study investigated if the combination of environmental and biological cues from aligned chitosan-poly-caprolactone (C-PCL) combined with TGF-β3 growth factor can efficiently and rapidly direct the tenogenic differentiation of primary human bone marrow stem cells (BMSCs). C-PCL nanofibers were prepared to have the anisotropic nanostructure, and mechanical and biological properties similar to those of the native tendon extracellular matrix (ECM). The tenogenic commitment of BMSCs was assessed using cell morphology, and gene and protein expressions. BMSCs grown on uniaxially aligned C-PCL nanofibers in a medium containing TGF-β3 displayed an elongated morphology along nanofiber orientation, upregulated expressions of marker genes, and increased collagen production associated with tenogenic differentiation as compared to control substrates. Significantly, this tenogenic microenvironment induced the transcription of tenogenic markers in 5 days and production of a large amount of Collagen I in 10 days, more effective and faster than existing scaffolds combined with growth factors. This research reveals that a combinative effect of aligned C-PCL nanofibers and TGF-β3, as environmental and biological cues, can lead to rapid, effective BMSC differentiation into tenogenic progenitors, offering a potential strategy for managing tendon disorders.

AB - Tendon injury occurs frequently and tendon repair is limited by its poor self-healing. The current tissue engineering approach for treating tendon injuries has showed limited success, largely due to the lack of scaffolds with suitable structural and biological properties, and suitable growth factors for differentiation of stem cells into tendon cells. This study investigated if the combination of environmental and biological cues from aligned chitosan-poly-caprolactone (C-PCL) combined with TGF-β3 growth factor can efficiently and rapidly direct the tenogenic differentiation of primary human bone marrow stem cells (BMSCs). C-PCL nanofibers were prepared to have the anisotropic nanostructure, and mechanical and biological properties similar to those of the native tendon extracellular matrix (ECM). The tenogenic commitment of BMSCs was assessed using cell morphology, and gene and protein expressions. BMSCs grown on uniaxially aligned C-PCL nanofibers in a medium containing TGF-β3 displayed an elongated morphology along nanofiber orientation, upregulated expressions of marker genes, and increased collagen production associated with tenogenic differentiation as compared to control substrates. Significantly, this tenogenic microenvironment induced the transcription of tenogenic markers in 5 days and production of a large amount of Collagen I in 10 days, more effective and faster than existing scaffolds combined with growth factors. This research reveals that a combinative effect of aligned C-PCL nanofibers and TGF-β3, as environmental and biological cues, can lead to rapid, effective BMSC differentiation into tenogenic progenitors, offering a potential strategy for managing tendon disorders.

UR - http://www.scopus.com/inward/record.url?scp=84887719661&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887719661&partnerID=8YFLogxK

U2 - 10.1039/c3tb20825g

DO - 10.1039/c3tb20825g

M3 - Article

AN - SCOPUS:84887719661

VL - 1

SP - 6516

EP - 6524

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

SN - 2050-7518

IS - 47

ER -