Temporal sequence of major biochemical events during Blood Bank storage of packed red blood cells

Brad S. Karon, Camille M. Van Buskirk, Elizabeth A. Jaben, James Hoyer, David D. Thomas

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background. We used sensitive spectroscopic techniques to measure changes in Band 3 oligomeric state during storage of packed red blood cells (RBC); these changes were compared to metabolic changes, RBC morphology, cholesterol and membrane protein loss, phospholipid reorganisation of the RBC membrane, and peroxidation of membrane lipid. The aim of the study was to temporally sequence major biochemical events occurring during cold storage, in order to determine which changes may underlie the structural defects in stored RBC. Materials and methods. Fifteen RBC units were collected from normal volunteers and stored under standard blood bank conditions; both metabolic changes and lipid parameters were measured by multiple novel assays including a new mass spectrometric measurement of isoprostane (lipid peroxidation) and flow cytometric assessment of CD47 expression. Band 3 oligomeric state was assessed by time-resolved phosphorescence anisotropy, and RBC morphology by microscopy of glutaraldehyde-fixed RBC. Results. Extracellular pH decreased and extracellular potassium increased rapidly during cold storage. Band 3 on the RBC membrane aggregated into large oligomers early in the storage period and coincident with changes in RBC morphology. Membrane lipid changes, including loss of unesterified cholesterol, lipid peroxidation and expression of CD47, also changed early during the storage period. In contrast loss of acetylcholinesterase activity and haemolysis of RBC occurred late during storage. Discussion. Our results demonstrate that changes in the macromolecular organisation of membrane proteins on the RBC occur early in storage and suggest that lipid peroxidation and/or oxidative damage to the membrane are responsible for irreversible morphological changes and loss of function during red cell storage.

Original languageEnglish (US)
Pages (from-to)453-461
Number of pages9
JournalBlood Transfusion
Volume10
Issue number4
DOIs
StatePublished - Nov 30 2012

Fingerprint

Blood Banks
Erythrocytes
Lipid Peroxidation
Membrane Lipids
Membrane Proteins
Cholesterol
Cell Membrane
Isoprostanes
Anisotropy
Glutaral
Acetylcholinesterase
Hemolysis
Microscopy
Phospholipids
Healthy Volunteers
Potassium

Keywords

  • Band 3
  • Red blood cell
  • Storage lesion

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology

Cite this

Temporal sequence of major biochemical events during Blood Bank storage of packed red blood cells. / Karon, Brad S.; Van Buskirk, Camille M.; Jaben, Elizabeth A.; Hoyer, James; Thomas, David D.

In: Blood Transfusion, Vol. 10, No. 4, 30.11.2012, p. 453-461.

Research output: Contribution to journalArticle

Karon, Brad S. ; Van Buskirk, Camille M. ; Jaben, Elizabeth A. ; Hoyer, James ; Thomas, David D. / Temporal sequence of major biochemical events during Blood Bank storage of packed red blood cells. In: Blood Transfusion. 2012 ; Vol. 10, No. 4. pp. 453-461.
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