Temporal profiling of the secretome during adipogenesis in humans

Jun Zhong, Sarah A. Krawczyk, Raghothama Chaerkady, Hailiang Huang, Renu Goel, Joel S. Bader, G. William Wong, Barbara E. Corkey, Akhilesh Pandey

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Adipose tissue plays a key role as a fat-storage depot and as an endocrine organ. Although mouse adipogenesis has been studied extensively, limited studies have been conducted to characterize this process in humans. We carried out a temporal proteomic analysis to interrogate the dynamic changes in the secretome of primary human preadipocytes as they differentiate into mature adipocytes. Using iTRAQ-based quantitative proteomics, we identified and quantified 420 proteins from the secretome of differentiated human adipocytes. Our results revealed that the majority of proteins showed differential expression during the course of differentiation. In addition to adipokines known to be differentially secreted in the course of adipocyte differentiation, we identified a number of proteins whose dynamic expression in this process has not been previously documented. They include collagen triple helix repeat containing 1, cytokine receptor-like factor 1, glypican-1, hepatoma-derived growth factor, SPARC related modular calcium binding protein 1, SPOCK 1, and sushi repeat-containing protein. A bioinformatics analysis using Human Protein Reference Database and Human Proteinpedia revealed that of the 420 proteins identified, 164 proteins possess signal peptides and 148 proteins are localized to the extracellular compartment. Additionally, we employed antibody arrays to quantify changes in the levels of 182 adipokines during human adipogenesis. This is the first large-scale quantitative proteomic study that combines two platforms, mass spectrometry and antibody arrays, to analyze the changes in the secretome during the course of adipogenesis in humans.

Original languageEnglish (US)
Pages (from-to)5228-5238
Number of pages11
JournalJournal of Proteome Research
Volume9
Issue number10
DOIs
StatePublished - Oct 1 2010
Externally publishedYes

Fingerprint

Adipogenesis
Adipocytes
Proteins
Proteomics
Adipokines
Glypicans
Protein Databases
Cytokine Receptors
Calcium-Binding Proteins
Antibodies
Protein Sorting Signals
Computational Biology
Bioinformatics
Adipose Tissue
Mass Spectrometry
Collagen
Mass spectrometry
Fats
Tissue

Keywords

  • adipokine
  • metabolism
  • protein microarrays
  • quantitative proteomics
  • secretome

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Zhong, J., Krawczyk, S. A., Chaerkady, R., Huang, H., Goel, R., Bader, J. S., ... Pandey, A. (2010). Temporal profiling of the secretome during adipogenesis in humans. Journal of Proteome Research, 9(10), 5228-5238. https://doi.org/10.1021/pr100521c

Temporal profiling of the secretome during adipogenesis in humans. / Zhong, Jun; Krawczyk, Sarah A.; Chaerkady, Raghothama; Huang, Hailiang; Goel, Renu; Bader, Joel S.; Wong, G. William; Corkey, Barbara E.; Pandey, Akhilesh.

In: Journal of Proteome Research, Vol. 9, No. 10, 01.10.2010, p. 5228-5238.

Research output: Contribution to journalArticle

Zhong, J, Krawczyk, SA, Chaerkady, R, Huang, H, Goel, R, Bader, JS, Wong, GW, Corkey, BE & Pandey, A 2010, 'Temporal profiling of the secretome during adipogenesis in humans', Journal of Proteome Research, vol. 9, no. 10, pp. 5228-5238. https://doi.org/10.1021/pr100521c
Zhong J, Krawczyk SA, Chaerkady R, Huang H, Goel R, Bader JS et al. Temporal profiling of the secretome during adipogenesis in humans. Journal of Proteome Research. 2010 Oct 1;9(10):5228-5238. https://doi.org/10.1021/pr100521c
Zhong, Jun ; Krawczyk, Sarah A. ; Chaerkady, Raghothama ; Huang, Hailiang ; Goel, Renu ; Bader, Joel S. ; Wong, G. William ; Corkey, Barbara E. ; Pandey, Akhilesh. / Temporal profiling of the secretome during adipogenesis in humans. In: Journal of Proteome Research. 2010 ; Vol. 9, No. 10. pp. 5228-5238.
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