Temporal expression patterns of BMP receptors and collagen II (B) during periosteal chondrogenesis

Arunik Sanyal, Merry Jo Oursler, Victoria R. Clemens, Takumi Fukumoto, James S. Fitzsimmons, Shawn W. O'Driscoll

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Articular cartilage has a limited ability to repair itself. Periosteal grafts have chondrogenic potential and are used clinically to repair defects in articular cartilage. An organ culture model system for in vitro rabbit periosteal chondrogenesis has been established to study the molecular events of periosteal chondrogenesis in vitro. In this model, bone morphogenetic protein-2 (BMP2) mRNA expression was found to be upregulated in the first 12 h. BMPs usually transduce their signals through a receptor complex that includes type II and either type IA or type IB BMP receptors. Receptors IA and IB play distinct roles during limb development. We have examined the temporal expression patterns for the mRNAs of these receptors using our experimental model. The mRNA expression patterns of these three BMP receptors differed from one another in periosteal explants during chondrogenesis. When these explants were cultured under chondrogenic conditions (agarose suspension with TGF-β1 added to the media for the first 2 days), the expression of BMPRII mRNA and that of BMPRIA mRNA varied only slightly and persisted over a long time. In contrast, the expression of BMPRIB mRNA was upregulated within 12 h, peaked at day 5, and fell to a level that was barely detected beyond day 21. Moreover, the expression of BMPRIB mRNA preceded that of collagen type IIB mRNAs, a marker for matrix-depositing chondrocytes. These data support a role for coordinate expression of BMP2 and its receptors early during periosteal chondrogenesis.

Original languageEnglish (US)
Pages (from-to)58-65
Number of pages8
JournalJournal of Orthopaedic Research
Volume20
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Bone Morphogenetic Protein Receptors
Chondrogenesis
Collagen
Messenger RNA
Bone Morphogenetic Protein 2
Articular Cartilage
Type I Bone Morphogenetic Protein Receptors
Organ Culture Techniques
Chondrocytes
Sepharose
Suspensions
Theoretical Models
Extremities
Rabbits
Transplants

Keywords

  • BMP receptors
  • Chondrogenesis
  • Collagen II (B)
  • Periosteum

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Temporal expression patterns of BMP receptors and collagen II (B) during periosteal chondrogenesis. / Sanyal, Arunik; Oursler, Merry Jo; Clemens, Victoria R.; Fukumoto, Takumi; Fitzsimmons, James S.; O'Driscoll, Shawn W.

In: Journal of Orthopaedic Research, Vol. 20, No. 1, 2002, p. 58-65.

Research output: Contribution to journalArticle

Sanyal, Arunik ; Oursler, Merry Jo ; Clemens, Victoria R. ; Fukumoto, Takumi ; Fitzsimmons, James S. ; O'Driscoll, Shawn W. / Temporal expression patterns of BMP receptors and collagen II (B) during periosteal chondrogenesis. In: Journal of Orthopaedic Research. 2002 ; Vol. 20, No. 1. pp. 58-65.
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