TCR-inducible PLZF transcription factor required for innate phenotype of a subset of γδ T cells with restricted TCR diversity

Taras Kreslavsky; Adam K. Savage; Robin Hobbs; Fotini Gounari; Roderick Bronson; Pablo Pereira; Pier Paolo Pandolfi; Albert Bendelac; Harald Von Boehmer

doi:10.1073/pnas.0903895106

TCR-inducible PLZF transcription factor required for innate phenotype of a subset of γδ T cells with restricted TCR diversity

Taras Kreslavsky, Adam K. Savage, Robin Hobbs, Fotini Gounari, Roderick Bronson, Pablo Pereira, Pier Paolo Pandolfi, Albert Bendelac, Harald Von Boehmer

Immunology

Research output: Contribution to journal › Article › peer-review

Abstract

Some γδ and αβ T lymphocytes exhibit an "innate" phenotype associated with rapid cytokine responses. The PLZF transcription factor is essential for the innate phenotype of NKT cells. This report shows that PLZF is likewise responsible for the innate, NKT-like phenotype of Vγ1+Vδ6.3/Vδ6.4+ cells. TCR cross-linking induced PLZF expression in all polyclonal immature γδthymocytes, suggesting that agonist selection might be required for PLZF induction. Transgenic expression of Vγ1Vδ6.4 TCR was sufficient to support the development of large numbers of PLZF+ T cells, further supporting the importance of the TCR for PLZF induction. Interestingly, expression of this TCR transgene led to the development of spontaneous dermatitis.

Original language	English (US)
Pages (from-to)	12453-12458
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	106
Issue number	30
DOIs	https://doi.org/10.1073/pnas.0903895106
State	Published - Jul 28 2009

Keywords

Agonist selection
Innate T cells
Transgenes

ASJC Scopus subject areas

General

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10.1073/pnas.0903895106

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Kreslavsky, T., Savage, A. K., Hobbs, R., Gounari, F., Bronson, R., Pereira, P., Pandolfi, P. P., Bendelac, A., & Von Boehmer, H. (2009). TCR-inducible PLZF transcription factor required for innate phenotype of a subset of γδ T cells with restricted TCR diversity. Proceedings of the National Academy of Sciences of the United States of America, 106(30), 12453-12458. https://doi.org/10.1073/pnas.0903895106

TCR-inducible PLZF transcription factor required for innate phenotype of a subset of γδ T cells with restricted TCR diversity. / Kreslavsky, Taras; Savage, Adam K.; Hobbs, Robin et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 30, 28.07.2009, p. 12453-12458.

Research output: Contribution to journal › Article › peer-review

Kreslavsky, T, Savage, AK, Hobbs, R, Gounari, F, Bronson, R, Pereira, P, Pandolfi, PP, Bendelac, A & Von Boehmer, H 2009, 'TCR-inducible PLZF transcription factor required for innate phenotype of a subset of γδ T cells with restricted TCR diversity', Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 30, pp. 12453-12458. https://doi.org/10.1073/pnas.0903895106

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abstract = "Some γδ and αβ T lymphocytes exhibit an {"}innate{"} phenotype associated with rapid cytokine responses. The PLZF transcription factor is essential for the innate phenotype of NKT cells. This report shows that PLZF is likewise responsible for the innate, NKT-like phenotype of Vγ1+Vδ6.3/Vδ6.4+ cells. TCR cross-linking induced PLZF expression in all polyclonal immature γδthymocytes, suggesting that agonist selection might be required for PLZF induction. Transgenic expression of Vγ1Vδ6.4 TCR was sufficient to support the development of large numbers of PLZF+ T cells, further supporting the importance of the TCR for PLZF induction. Interestingly, expression of this TCR transgene led to the development of spontaneous dermatitis.",

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AU - Kreslavsky, Taras

AU - Savage, Adam K.

AU - Hobbs, Robin

AU - Gounari, Fotini

AU - Bronson, Roderick

AU - Pereira, Pablo

AU - Pandolfi, Pier Paolo

AU - Bendelac, Albert

AU - Von Boehmer, Harald

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N2 - Some γδ and αβ T lymphocytes exhibit an "innate" phenotype associated with rapid cytokine responses. The PLZF transcription factor is essential for the innate phenotype of NKT cells. This report shows that PLZF is likewise responsible for the innate, NKT-like phenotype of Vγ1+Vδ6.3/Vδ6.4+ cells. TCR cross-linking induced PLZF expression in all polyclonal immature γδthymocytes, suggesting that agonist selection might be required for PLZF induction. Transgenic expression of Vγ1Vδ6.4 TCR was sufficient to support the development of large numbers of PLZF+ T cells, further supporting the importance of the TCR for PLZF induction. Interestingly, expression of this TCR transgene led to the development of spontaneous dermatitis.

AB - Some γδ and αβ T lymphocytes exhibit an "innate" phenotype associated with rapid cytokine responses. The PLZF transcription factor is essential for the innate phenotype of NKT cells. This report shows that PLZF is likewise responsible for the innate, NKT-like phenotype of Vγ1+Vδ6.3/Vδ6.4+ cells. TCR cross-linking induced PLZF expression in all polyclonal immature γδthymocytes, suggesting that agonist selection might be required for PLZF induction. Transgenic expression of Vγ1Vδ6.4 TCR was sufficient to support the development of large numbers of PLZF+ T cells, further supporting the importance of the TCR for PLZF induction. Interestingly, expression of this TCR transgene led to the development of spontaneous dermatitis.

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TCR-inducible PLZF transcription factor required for innate phenotype of a subset of γδ T cells with restricted TCR diversity

Abstract

Keywords

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